北五加皮不同时间水提物中绿原酸含量的变化研究  

Study on the Content Change of the Chlorogenic Acid in Water Extract of Cortex periplocae at the Different Times

作　　者：关颖丽[1] 蔡黎明 杨荣艳[2] 

机构地区：[1]通化师范学院制药与食品科学系,吉林通化134002 [2]吉林省四平市食品药品检验所,吉林通化136001

出　　处：《北方园艺》2011年第10期154-155,共2页Northern Horticulture

基　　金：吉林省教育厅科研基金资助项目(吉教科合字[2009]第464号)

摘　　要：利用HPLC法研究了北五加皮不同时间水提物中绿原酸含量的变化规律。采用色谱柱Agilent ZORBAX SB-C18(150mm×4.6mm,5μm),流动相为0.4%磷酸溶液(A)-乙腈(B),梯度洗脱[0～8min 24%B;8～12min 50%B],检测波长278nm,流速为1.0mL/min,柱温为25℃。结果表明:绿原酸的最佳提取时间为2h,该方法简单快速、可行,含量测定准确。The content change of chlorogenic acid in water extract of Cortex periplocae at the different times were determined by HPLC.The ZORBAX SB-C18 columm(150 mm×4.6 mm,5μm)was used,the mobile phase was 0.4%phosphoric acid solution(A)-methyl cyanide(B),the gradient elution[0～8 min 24%B;8～12 min 50%B]. The flow rate was 1.0 mL/min,the detection wavelength was 278 nm.The temperature of column was 25℃.The results showed that two hours was the best time to extraction of chlorogenic acid.The method was simple,fast,feasible and accurate.

关 键 词：北五加皮 绿原酸 HPLC 

分 类 号：S6[农业科学—园艺学]