双价抗病虫基因植物表达载体Lc-ChiA-pBI121的构建  

Construction of Lc-ChiA-pBI121 Plant Expression Vector Containing Two Pest and Disease Resistant Genes

作　　者：田强强[1,2] 徐利[3] 谭黎霞 王敦[2] 李厚华[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100 [2]植保资源与病虫害治理教育部重点实验室,陕西杨凌712100 [3]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《北方园艺》2011年第11期101-103,共3页Northern Horticulture

基　　金：西北农林科技大学国外引进人才科研启动基金资助项目(Z111020901);陕西省自然科学基金资助项目(2010JQ3009)

摘　　要：采用35S启动子将抗病基因玉米Lc转录因子和抗病虫基因棉铃虫核型多角体病毒(HearNPV)的几丁质酶(ChiA),连接到高效植物表达载体pBI121中,进行酶切鉴定。结果表明:含有上述2个基因,且转录方向相同的双价植物表达载体已经构建成功;含有上述2个基因的双价载体国内外尚未见报道,用于植物转基因研究,可提高植物抗病虫害能力。The maize Lc transcription factor gene having an effect on disease-resistance and the HearNPV chitinase gene having both disease-resistant and insect-resistant abilities were ligated into the plant expression vector,pBI121.It was confirmed by the restriction enzyme digestion that the recombinant vector,Lc-ChiA-pBI121,was constructed successfully with the same transcriptional direction.The plant expression vector containing these two genes could be used for plant transgenic research to enhance plant resistance to deseases and pest.

关 键 词：植物表达载体 构建 Lc转录因子 几丁质酶 

分 类 号：S6[农业科学—园艺学]