用改良Hodge试验检测产碳青霉烯酶肠杆菌科细菌  

Detection of carbapenemase in isolates of Enterobacteriaceae by the modified Hodge test

作　　者：杨燕[1] 孙长贵[1] 陈坚[1] 熊敏[1] 成军[1] 

机构地区：[1]解放军117医院检验科,浙江杭州310013

出　　处：《东南国防医药》2011年第3期223-225,共3页Military Medical Journal of Southeast China

摘　　要：目的了解分离的肠杆菌科细菌产碳青霉烯酶情况,为临床正确选择抗菌药物提供依据。方法采用K-B纸片扩散法检测并筛选厄他培南和美罗培南,以抑菌环直径分别为19~21 mm和16~21 mm的菌株作为待测菌,进行改良的Hodge试验。结果 390株肠杆菌科细菌中筛选出55株可疑产碳青霉烯酶菌株,对可疑菌株进行改良的Hodge试验,阳性率21.82%(12/55)。结论碳青霉烯类抗生素敏感折点附近的肠杆菌科细菌有近1/5菌株产碳青霉烯酶,临床微生物学实验室应执行改良Hodge试验,以确保药敏试验结果的准确性。Objective To investigate carbapenemase production in isolates of Enterobacteriaceae isolated from our hospital and provide a correct choice of antibiotic for the clinician.Methods Antibiotic susceptibility test was performed by K-B method.Strains showing zones of inhibition 19-21 mm with Ertapenem or 16-21 mm with Meropenem were screened as suspicious carbapenemase producing ones and tested for carbapenemase by the modified Hodge test.Results 55 of 390 strains of Enterobacteriaceae were suspected of possessing carbapenemases,which revealed 21.82% positive rate in the modified Hodge test.Conclusion Nearly one-fifth of strains of Enterobacteriaceae whose carbapenems zones of inhibition were nearby susceptible break point generated carbapenemase.The modified Hodge test should be done to ensure the accuracy of the sensitivity test.

关 键 词：肠杆菌科 碳青霉烯酶 抗生素 敏感性 

分 类 号：R9[医药卫生—药学]