牙鲆sox9基因的克隆与表达研究  

Study on Cloning and Expression of Gene sox9 in Olive Flounder(Paralichthys olivaceus)

作　　者：文爱韵[1,2] 尤锋[1] 孙鹏[1] 徐冬冬[1] 吴志昊[1] 马得友[1] 李军[1] 张培军[1] 

机构地区：[1]中国科学院海洋研究所海洋生物学重点实验室,山东青岛266071 [2]中国科学院研究生院,北京100039

出　　处：《海洋科学进展》2011年第1期97-104,共8页Advances in Marine Science

基　　金：国家重点基础研究发展计划项目--重要养殖鱼类功能基因组的分子设计育种的基础研究(2010CB126304);山东省自然科学基金项目项目--牙鲆P450arom基因及其转录因子在其性别表型形成中作用的研究(ZR2010CM007);中国科学院实验海洋生物学重点实验室开放基金项目--牙鲆性腺发生及性别表型形成的初步研究(kf201010)

摘　　要：通过Genome walking方法克隆得到了牙鲆(Paralichthys olivaceus)sox9的基因组全长序列,对其在成鱼各组织的差异表达进行了RT-PCR分析。结果显示:牙鲆sox9基因全长3 573 bp,包含3个外显子和2个内含子,编码的蛋白全长478 aa,含有高度保守的HMG结构域;sox9基因在性腺的表达具有两性差异,精巢中的表达明显强于卵巢中的表达,属1个性别相关基因,但其在肝脏、心脏、鳃、脑、眼和胃等多种组织中也有不同程度的表达。据此进一步分析和讨论等sox9基因在牙鲆性腺发育中的作用。Full sequence of flounder(Paralichthys olivaceus) Gene sox9 is cloned by means of the genome walking method.The differonce among the gene expressions of the various tissues of the adult is analyzed by use of RT-PCR.It is learnt from the anlysis results that the total length of Gene sox9 is 3 573 bp,including three exons and two introns.The encoded protein is 478 aa long with a highly conserved HMG structural domain.Gene sox9 expression is sex-related,in the other words sexual dimorphous in the gonads.Its expression is more obvious in testis than in ovary.Additionally,there are some differences among the Gene sox9 expressions for the other tissues such as liver,heart,gill,brain,eye,stomach etc.Finally the possible role of Gene sox9 in flounder gonadal development is discussed and analyzed.

关 键 词：牙鲆(Paralichthys olivaceus) SOX9 基因克隆 两性差异表达 

分 类 号：P76[天文地球—海洋科学]