吸烟诱导慢性阻塞性肺疾病患者痰及肺组织β-防御素2表达  

Expression of Beta-defensin-2 in Sputum and Lung Tissue of Nonsmokers and Smokers with or without Chronic Obstructive Pulmonary Disease

作　　者：胡瑞成 谭双香 欧阳卿 戴爱国 

机构地区：[1]湖南省老年医院-湖南省老年医学研究所,湖南长沙410016

出　　处：《中国呼吸与危重监护杂志》2011年第3期215-219,共5页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：湖南省自然科学基金(编号:06JJ4056);湖南省医药卫生科研基金(编号:C2006-038;B2008-026)

摘　　要：目的初步探讨吸烟对诱导痰和肺组织β-防御素2(BD-2)表达水平的影响及其与慢性阻塞性肺疾病(COPD)的关系。方法将早期周围型肺鳞癌拟行肺叶切除的患者分为COPD吸烟组(COPD组)、非COPD吸烟组(吸烟组)和不吸烟组(对照组)。术前采用高渗盐水雾化吸入诱导收集痰液,术中收集肿瘤远处肺组织标本,对痰液进行细胞分类计数,HE染色观察肺组织病理学形态,ELISA方法测定痰和肺组织匀浆中的BD-2浓度,对BD-2与吸烟指数、气道炎症指标、肺通气功能指标进行直线相关分析。结果研究对象的肺组织病理学形态与试验分组高度吻合。对照组痰细胞总数、痰中性粒细胞比例、肺组织匀浆BD-2浓度分别为(2.32±0.51)×106/g、(35.7±9.8)%、(14.5±5.7)ng/L,吸烟组和COPD组痰细胞总数、痰中性粒细胞比例、肺组织匀浆BD-2浓度则分别为(4.57±0.87)×106/g、(52.5±10.9)%、(78.3±13.1)ng/L和(6.61±1.03)×106/g、(65.5±12.3)%、(127.0±35.0)ng/L,以上3项指标在对照组、吸烟组、COPD组依次增高(P<0.05)。吸烟组和COPD组痰淋巴细胞比例、痰BD-2浓度分别为(2.5±1.2)%、(315.0±124.0)ng/L和(3.2±1.7)%、(298.0±135.0)ng/L,以上2项指标两组之间比较差异无统计学意义(P>0.05),但均高于对照组[(1.1±0.3)%、(132.0±48.0)ng/L](P<0.05)。直线相关分析结果显示痰BD-2浓度与吸烟指数、痰细胞总数及中性粒细胞比例呈正相关,而肺组织匀浆BD-2浓度与肺通气功能呈负相关(P<0.05)。结论吸烟导致痰液和肺组织BD-2水平升高,肺组织BD-2表达状况可能与吸烟个体的COPD易感性相关。Objective To investigate the effects of smoking on β-defensin-2(BD-2) expression in induced sputum and lung tissue,and its role in chronic obstructive pulmonary disease(COPD).Methods Patients suffering with early peripheral squamous celled lung cancer and underwent lobectomy were divided into a smoking COPD group(COPD group),a non-COPD smoking group(smoker group),and a non-smoking group(control group).Preoperative induced sputum samples were collected after hypertonic saline induction.Lung tissue samples were intraoperatively collected far from the tumor site.The sputum samples were prepared for total and differential cell count,while the lung tissue samples for pathology examination.The BD-2 concentration in sputum and lung homogenate were measured by ELISA.Correlation were analyzed between BD-2 concentration and smoking index,airway inflammation,and lung function.Results The lung pathology were highly consistent with the experimental grouping.The total cell count and neutrophils proportion in sputum and BD-2 concentration in lung homogenate were(2.32±0.51)×106/g,(35.7±9.8)%,and(14.5±5.7)ng/L in the control group respectively,while increased in the smoker group [(4.57±0.87)×106/g,(52.5±10.9)%,and(78.3±13.1)ng/L,P<0.05],and further increased in the COPD group [(6.61±1.03)×106/g,(65.5±12.3)%,and(127.0±35.0)ng/L,P<0.05].The lymphocytes proportion and BD-2 concentration in sputum increased in the COPD group [(3.2±1.7)% and(298.0±135.0)ng/L] as well as in the smoker group [(2.5±1.2)% and(315.0±124.0)ng/L],as compared with the control group [(1.1±0.3)% and(132.0±48.0)ng/L](P<0.05).Linear correlation analysis revealed that BD-2 concentration in sputum was positively correlated with smoking index,sputum total cell count and neutrophils proportion,whereas BD-2 concentration in lung homogenate was reversely correlated with pulmonary ventilation function(P<0.05).Conclusions Smoking up-regulates the BD-2 level in sputum and lung tissues.Further more,the BD-2 expression status in lung tissue of smoking ind

关 键 词：慢性阻塞性肺疾病 吸烟 Β-防御素2 痰 

分 类 号：R563.9[医药卫生—呼吸系统]