丹参酮ⅡA磺酸钠在减轻海水淹溺性肺水肿中的作用及机制  

Effect and Mechanism of Sodium Tanshinone ⅡA Sulphonate in Attenuating Acute Pulmonary Edema of Seawater Drowning

作　　者：谢晓燕[1,2] 张博[3] 李佳欢[1] 范启新[1] 张勇[1] 金发光[1] 

机构地区：[1]第四军医大学唐都医院呼吸科,陕西西安710038 [2]解放军第451医院呼吸科,陕西西安710054 [3]第四军医大学病理生理教研室,陕西西安710032

出　　处：《中国呼吸与危重监护杂志》2011年第3期252-256,共5页Chinese Journal of Respiratory and Critical Care Medicine

摘　　要：目的探讨丹参酮ⅡA磺酸钠(STS)是否可减轻海水淹溺性肺水肿(PE-SWD)及其对钠钾ATP酶(NKA)的作用。方法将大鼠随机分为正常组、海水组和STS治疗组。应用海水吸入法建立大鼠PE-SWD模型。检测海水灌注前,海水灌注后30 min、1 h、2 h、4 h和6 h的PaO2。在造模4 h后,观察肺组织形态学改变、测定肺湿/干重比值(W/D)、肺毛细血管通透性(PMVP)及肺组织匀浆中NKA活性,采用Western blot检测NKAα1亚基蛋白表达。结果海水吸入导致PaO2和NKA表达及活性均下降,使W/D、PMVP增加。STS治疗组从海水灌注后2 h开始PaO2显著高于海水组[(67.58±3.45)mm Hg比(59.00±3.86)mm Hg,P<0.05)],到4 h PaO2值达高峰[(80.75±3.57)mm Hg比(70.5±4.48)mm Hg,P<0.01)];肺组织形态学表明海水组肺组织出血、水肿,肺泡间隔明显增厚,炎性细胞渗入肺泡腔;STS治疗组肺损伤明显减轻;STS治疗组W/D较海水组明显下降(5.53±0.45比6.40±0.42,P<0.05),PMVP值STS治疗组亦较海水组明显减轻[(52.75±5.86)μg/g比(65.63±6.34)μg/g,P<0.05];肺组织匀浆中NKA活性及NKA-α1亚基蛋白表达STS治疗组均较海水组明显增高(P<0.05)。结论 STS能够减轻海水淹溺性肺水肿,此作用可能与其增加NKA活性及表达有关。Objective To investigate whether sodium tanshinone ⅡA sulphonate(STS) treatment attenuates pulmonary edema of seawater drowning(PE-SWD),and examine the effects of STS on Na-K-ATPase(NKA) in PE-SWD.Methods Thirty-six rats were randomly divided into there groups,ie.a normal group(NG),a seawater group(SG),and a STS treatment group(TG).The rat model of PE-SWD was established by seawater instillation.PaO2,histological changes of lungs,lung wet/dry weight ratio(W/D),pulmonary microvascular permeability(PMVP),and NKA activity were detected.Western blot were used to test the effects of STS on NKAα1 expression.Results Seawater instillation decreased PaO2 and the expression of NKA,while increased W/D ratio and PMVP.At 2 h after seawater instillation,the PaO2 in the TG group were significantly higher than those in the SG group,and peaked at 4 h after seawater instillation.Histological examination showed that there were hemorrhage,edema,markedly thickened alveolar wall,and infiltration of inflammatory cells in alveolar spaces in the SG group,but lung injury was significantly alleviated in the TG group.W/D ratio and PMVP in the TG group were significantly lower than those in the SG group.Additionally,NKA activity and NKA-α1 expression were significantly higher in the TG group than those in the SG group.Conclusion STS treatment can attenuate pulmonary edema of seawater drowning which may be related with up-regulating Na-K-ATPase activity and expression.

关 键 词：急性肺水肿 海水 淹溺 丹参酮ⅡA磺酸钠 钠钾ATP酶 

分 类 号：R285.5[医药卫生—中药学]