矽肺患者检测血清转化生长因子-β_1和肿瘤坏死因子-α意义研究  

Research on the detection of serum TGF-β_1 and TNF-αin silicosis patients

作　　者：缪荣明 赵道昆 张雪涛[2] 张颖轶 郭平 杨志刚 陈洁 姚明莺 赵锐 房中华 

机构地区：[1]无锡市职业病防治医院,214028 [2]上海市杨浦区中心医院,200090

出　　处：《中国疗养医学》2011年第8期731-732,共2页Chinese Journal of Convalescent Medicine

基　　金：上海市卫生局科研课题(2007029);无锡市卫生科研基金项目(XM0909)

摘　　要：目的观察矽尘接触者和矽肺患者的血清转化生长因子-β1(TGF-β1)和肿瘤坏死因子-α(TNF-α),探讨TGF-β1、TNF-α在矽肺发生发展中的作用。方法采用ELISA法对100名不接触粉尘的对照人群,200名接触矽尘1年以上的接尘工人,32名矽尘作业观察对象(原0+患者)及130例矽肺患者,检测其血清TGF-β1、TNF-α水平。结果与对照组对照:接尘组、观察对象组和矽肺组TNF-α水平明显高于正常对照组,差异有高度统计学意义(P<0.01);与接尘组对照:矽肺组TNF-α水平明显高于接尘组(P<0.01),观察对象组高于接尘组(P<0.05);与对照组及接尘组对照:TGF-β1矽肺组明显高于正常对照组及接尘组,差异有高度统计学意义(P<0.01)。结论外周血TGF-β1、TNF-α水平与肺纤维化的发生发展关系紧密,检测其表达水平对职业健康监护、职业病诊断、治疗提供了有力的依据。Objectives To observe the change of serum TGF-β_1 and TNF-α in silicosis patients and workers exposed to silica dust to study the role of TGF-β_1 and TNF-α in the development of silicosis.Methods One hundred non-exposed workers were selected as control group,200 workers exposed to silica dust for more than 1 year as exposed group,32 suspected silicosis patients(originally diagnosed as 0+)as observing group,130 silicosis patients were as silicosis group.Serum TGF-β_1 and TNF-α in each group were determined with ELISA.Results Serum TNF-α in exposed group,observing group,silicosis group were significantly higher than that in control group(P <0.01).Silicosis group and observing group were also higher than exposed group(P <0.01,P <0.05).Compared with control group and exposed group,serum TGF-β_1 in silicosis group was much higher(P<0.01).Conclusion TGF-β_1 and TNF-α in peripheral blood was closely related to pulmonary fibrosis,so it is a powerful evidence for the prevention,diagnosis and treatment for silicosis.

关 键 词：矽肺 转化生长因子-Β1 肿瘤坏死因子-Α 

分 类 号：R563.9[医药卫生—呼吸系统]