Cnidilin的微生物转化及其代谢产物的结构鉴定  

Microbial Transformation of Cnidilin and Identification of Its Metabolites

作　　者：朱红[1] 张兰桐[1] 任艳平[1] 许慧君[1] 崔洋[1] 

机构地区：[1]河北医科大学药学院药物分析教研室,河北石家庄050017

出　　处：《中国医药工业杂志》2011年第5期336-339,共4页Chinese Journal of Pharmaceuticals

基　　金：河北省科技支撑计划项目(09276423D)

摘　　要：采用微生物转化法进行cnidilin(8-甲氧基异欧前胡内酯)的体外代谢研究。选取20种真菌对cnidilin进行体外转化,采用HPLC-PDA检测,显示了4个代谢物,将其中的两个放大制备,经1H NMR和ESI-MS推断分子结构,这两个代谢产物为3"-甲氧基-8-甲氧基异欧前胡内酯和5"-羟基-8-甲氧基异欧前胡内酯。The in vitro metabolism of cnidilin(8-methoxyisoimperatorin) was studied by the microbial transformation method.Twenty fungal strains were chosen for the in vitro transformation of cnidilin.An HPLC-PDA method was used for determination and four metabolites were discovered.Two of them were prepared and identified by 1H NMR and ESI-MS,which are 3'-methoxy-8-methoxyisoimperatorin and 5'-hydroxyl-8-methoxyisoimperatorin.

关 键 词：cnidilin 8-甲氧基异欧前胡内酯 微生物转化 体外代谢 结构解析 

分 类 号：R9[医药卫生—药学]