电针对6-羟多巴胺毁损大鼠黑质铁含量变化的影响  

Effect of electroacupuncture on the iron levels in the substantia nigra of 6-OHDA lesioned rat

作　　者：于永鹏[1] 丛海铭[1] 丛艳彬[1] 毕可萍[1] 

机构地区：[1]潍坊医学院附属威海市文登中心医院神经内科,山东省264400

出　　处：《中华临床医师杂志（电子版）》2011年第8期2309-2312,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：威海市科技发展计划项目(2010-3-28-4)

摘　　要：目的探讨电针疗法治疗帕金森病(PD)神经保护作用的机制。方法采用6-羟多巴胺(6-OHDA)偏侧黑质毁损大鼠模型,实验大鼠分为正常组、正常电针组、PD模型组、电针组、假电针组,每组9只大鼠。大鼠进行电针处理2周后,利用原子吸收分光光度法测定各组大鼠黑质铁含量,观察针刺某些特定穴位对偏侧毁损大鼠脑黑质铁含量的影响。结果 PD模型组大鼠黑质铁含量较正常组明显升高(P<0.05),旋转行为学明显异常;而电针组大鼠黑质铁含量较PD模型组显著减少(P<0.05),旋转行为学明显改善。结论电针疗法可以降低6-OHDA偏侧毁损大鼠脑黑质内铁的含量,改善大鼠的旋转行为学,研究结果提示电针疗法对6-OHDA偏侧毁损大鼠脑黑质内铁代谢有调节作用,这也可能是针刺治疗PD神经保护作用的机制之一。Objective To explore the mechanism of the neuroprotective effect of electroacupuncture in the treatment of Parkinson's disease(PD).Methods The rat model of PD with a unilateral injection of 6-OHDA into SNc was adopted.Fifty five healthy male Wistar rats were divided into five groups randomly,normal group,PD group,normal-electroacupuncture group,electroacupuncture group,sham-electroacupuncture group,control group,siRNA control group,and nine rats per group.Rats in each group except normal group were treated with electroacupuncture for two weeks.The effect of electroacupuncture in some special acupoints on the content of iron in the substantia nigra of rat,which was determined by atomic absorption spectrometry(AAS),was investigated.Result Compared with the normal group,the levels of iron in the PD group were significantly higher as well as the net turns of unilateral lesioned rats do.However,compared with the PD group,the levels of iron in the electroacupuncture group were significantly decreased companied with the improvement of rotational behavior.Conclusions Electroacupuncture could decrease the content of iron in the substantia nigra of 6-OHDA unilateral lesioned rat and improve its rotational behavior.The Results in the present study reveal that electroacupuncture may have effect on the regulation in the metabolism of iron in the substantia nigra of 6-OHDA unilateral lesioned rat.It may be one of the mechanisms on the neuroprotective effect of electroacupuncture in the PD treatment.

关 键 词：帕金森病 羟多巴胺 电针 铁 

分 类 号：R245[医药卫生—针灸推拿学]