普罗布考对实验型大鼠胱抑素C、肾功能和肾脏病理学的影响  

Effect of Probucol on cystatin C,renal function and renal pathology in experiment rat

作　　者：杨成[1] 兰天飙[1] 

机构地区：[1]广州军区武汉总医院肾内科,430070

出　　处：《中华临床医师杂志（电子版）》2011年第9期2582-2586,共5页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的观察普罗布考对实验型大鼠血清胱抑素C(CysC)、肾功能及肾脏病理学的影响,并探讨CysC对肾小球肾病的诊断价值。方法雄性SD大鼠40只,随机分为正常组、模型组、治疗组。尾静脉一次性注射阿霉素6mg/kg制备阿霉素肾病模型。1周后开始药物干预,持续4周。检测大鼠24h尿蛋白、血清CysC、尿素氮(BUN)、血清肌酐(Scr)、血清和肾组织中血清超氧化物歧化酶(SOD)和丙二醛(MDA),同时取肾组织标本观察病理学改变。结果治疗组24h尿蛋白排泄量明显低于模型组(P<0.05),治疗组CysC明显降低(P<0.05),肾功能明显好转,肾指数明显改善(P<0.05),血清和肾组织中SOD明显上升、MDA明显下降(P<0.05),肾组织病理损伤减轻。结论普罗布考能减少实验型大鼠蛋白尿,降低CysC,减轻肾组织的病理损伤,具有肾保护作用。CysC对肾小球肾病的早期诊断有一定的价值,能较Scr更早反映肾功能的变化。Objective To investigate the effects of Probucol on cystatin C(Cys C),renal function and renal pathology in rats with adriamycin-induced nephropathy,and explore the diagnostic value of Cys C in glomcrulus nephropathy.Methods Forty male SD rats were used.The rats were randomly divided into three groups:normal group,model group and treated group.The models of adriamycin-induced nephropathy were established by injection of 6mg/kg adriamycin into the tail vein.After 1 week,medicine interventions were used for 4 weeks.The level of 24-hour urinary protein and blood biochemistry indexs were measured,and renal pathologic changes were examined.Results In the treated group,24-hour urinary protein,Cys C levels were significantly decreased(P<0.05),renal function was significantly improved,renal index was significantly improved.The SOD activity were significantly increased and the MDA were significantly decreased(P<0.05),renal pathologic changes were improved.Conclusions In rats with adriamycin-induced nephropathy,Probucol can decrease the urinary protein and Cys C,improve the renal pathologic changes.It has protective effects.Serum Cys C may be good biomarkers for early diagnosis of glomcrulus nephropathy and they can reflect the change of renal function after contrast injection earlier than serum creatinine.

关 键 词：普罗布可 大鼠 裸 肾病 

分 类 号：R965[医药卫生—药理学]