早老素相关菱形样蛋白基因Leu262Val多态性与2型糖尿病及糖尿病肾病的相关性研究  

Association between the Leu262Val polymorphism of presenilin associated rhomboid like protein (PARL) and type 2 diabetes and diabetic nephropathy paitents

出　　处：《中华临床医师杂志（电子版）》2011年第10期2824-2828,共5页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(30760087); 云南省自然科学基金(2004C0066m)

摘　　要：目的探讨早老素相关菱形样蛋白(PARL)基因Leu262Val多态性与2型糖尿病(T2DM)及糖尿病肾病(DN)的相关性。方法运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,在昆明地区汉族人中对278例T2DM患者[其中正常白蛋白尿(DN0)组102例,微量白蛋白尿(DN1)组91例,临床白蛋白尿(DN2)组85例]和113例健康对照者(NC)的PARL基因Leu262Val多态性进行检测,并比较分析各组间基因型频率和等位基因频率以及相关临床资料。结果 T2DM组和NC组间,DN组(DN1+DN2)、DN0组和NC组各组间基因型频率及等位基因频率差异均无统计学意义(P>0.05),二分类Logistic回归分析表明:在T2DM患者中,DM病程、收缩压(SBP)、甘油三酯(TG)和糖化血红蛋白百分比(HbA1c%)是DN发生的危险因素。结论在昆明地区汉族人中PARL基因Leu262Val多态性可能与T2DM及DN的发生无相关性;DM病程、SBP、TG、HbA1c可能是DN的危险因素。 Objective To investigate the association of the Leu262Val polymorphism of presenilin associated rhomboid like protein(PARL) with type 2 diabetes mellitus(T2DM) and diabitic nephropathy in Kunming Han Chinese.Methods The Leu262Val polymorphism of PARL was detected by PCR-RFLP in 278 unrelated Kunming Han patients with T2DM,and 113 normal controls(NC).There are 102 normal albuminuria(DN0 group),91 microalbuminuria(DN1 group) and 85 macroalbuminuria(DN2 group) among T2DM.The genotype and allele frequencies and relative clinical data were compared among these groups.Results There were no significant differences in genotype frequencies or allele frequencies not only between T2DM and NC but also among the three groups(DN,DN0 and NC)(P>0.05).Logistic regression analysis indicated that diabetic duration,SBP,TG,HbA1c% were risk factors of type 2 Diabetic nephropathy.Conclusions The Leu262Val polymorphism of PARL may not be risk factor of T2DM and DN progression.The diabetic duration,SBP,TG and HbA1c% may be risk factors of DN progression of Han Chinese in Kunming.

关 键 词：糖尿病 2型 糖尿病肾病 早老素类 多态性 单核苷酸 

分 类 号：R587.2[医药卫生—内分泌]