华蟾素对MDA-MB-231细胞线粒体膜电位及活性氧的影响  

Effects of Cinobufacini on Reactive Oxygen Species and Mitochondrial Membrane Potential in MDA-MB-231 Cells

作　　者：马丽娜[1] 刘志刚[2] 宋兵[3] 彭元[1] 蔡继业[1] 金花[1] 陈辉[2] 

机构地区：[1]暨南大学化学系,广东广州510632 [2]暨南大学水生生物研究所,广东广州510632 [3]暨南大学组织移植与免疫实验中心,广州广东510632

出　　处：《生物技术》2011年第6期35-38,共4页Biotechnology

基　　金：国家重点基础研究发展计划(973计划)项目(No.2010CB833603);国家自然科学基金项目(No.30872404)资助

摘　　要：目的:研究华蟾素诱导乳腺癌MDA-MB-231细胞凋亡过程中,细胞内活性氧(ROS)及线粒体膜电位(△Ψm)的变化,探讨华蟾素对乳腺癌细胞的作用机制。方法:用不同浓度的华蟾素作用于MDA-MB-231细胞24h后,分别用荧光探针罗丹明123和荧光探针DCFH-DA进行荧光染色,用流式细胞仪检测细胞内线粒体膜电位和活性氧的变化。结果:不同浓度的华蟾素作用于MDA-MB-231细胞后,随着药物浓度的增加(0、12.5、25、37.5、50μg/ml),细胞内的ROS水平显著升高,荧光强度从3 609±24上升为6 263±35;同时,线粒体膜电位(△Ψm)显著下降,荧光强度从242±6降低到173±4。结论:华蟾素作用细胞后,使得细胞内活性氧水平显著升高,同时,线粒体膜电位显著下降,推测华蟾素对MDA-MB-231细胞通过线粒体途径诱发细胞凋亡。Objective:To explore the effect of cinobufacini on the production of reactive oxygen species(ROS) and mitochondrial membrane potential in human cancer cell line MDA-MB-231.Method:The MDA-MB-231 cells treated with different concentrations of cinobufacini for 24 h,then used probe rhodamine-123 as mitochondrial membrane potential probe and used DCFH-DA probe as ROS probe,the levels of mitochondrial membrane potential and ROS of MDA-MB-231cells were tested by flow cytomentry.Result:After treated with different concentrations of cinobufacini,the ROS levels were significantly higher than control group and the mitochondrial membrane potential levels were lower than control groups significantly.Conclusion:Cinobufacini could increase the cytosolic ROS levels in MDA-MB-231cells and decrease mitochondrial membrane potential levels induced mitochondrial apoptosis pathway.

关 键 词：华蟾素 MDA-MB-231细胞 活性氧(ROS) 线粒体膜电位 凋亡 

分 类 号：Q81[生物学—生物工程]