诱导溶源性嗜盐古生菌产生噬菌体的方法  

A Method of Inducing Lysogenic Haloarchaea to Release Halophage

作　　者：梅运军[1] 陈锦 沈萍[2] 

机构地区：[1]武汉工业学院化学与环境工程学院,湖北武汉430023 [2]武汉大学生命科学学院,湖北武汉430074

出　　处：《生物技术》2011年第6期57-60,共4页Biotechnology

基　　金：国家自然基金项目(3087013)资助

摘　　要：目的:用简单易行的诱导手段,从溶源性的嗜盐古生菌中诱导产生新的噬菌体,为分离嗜盐古生菌噬菌体提供一种新的途径。方法:分别用紫外线与丝裂霉素C对10株对数期的嗜盐古生菌菌株进行诱导,上清液采用双层平板法进行噬菌斑鉴定,并用脉冲场凝胶电泳对噬菌体基因组进行分析。结果:经1μg/mL丝裂霉素C诱导的嗜盐古生菌融合子F5产生了一株新的嗜盐古生菌噬菌体SNJ1,该噬菌体能感染Natrinema属的菌株J7。结论:丝裂霉素C能诱导原噬菌体从宿主中分离,为嗜盐古生菌噬菌体分离提供了一条新的途径。Objective:An easily method was presented to induce lysogenic haloarchaea to release phage,which provided a new approach to isolate halophage.Method:Ultraviolet and mitomycin C were employed to induce ten logarithmic phase haloarchaeal strains,plaque was assayed through double-layer agar plates and the genome of halophage was detected by pulsed-field gel electrophoresis.Result:Only trans-genus fusant F5 was able to produce halopahge progeny SNJ1 by induction with 1 μg/mL mitomycin C which infected Natrinema sp.J7.Conclusion:Mitomycin C was able to induce lysogenic haloacrchaea to release halophage,which presented a new method to isolate halohage.

关 键 词：嗜盐古生菌 噬菌体 嗜盐古生菌噬菌体 原噬菌体 

分 类 号：Q81[生物学—生物工程]