芒果苷对过氧化氢诱导的晶状体氧化损伤的保护作用  

The antioxidative effect of mangiferin on the lens

作　　者：吴雪 杨东[2] 葛红岩[2] 刘平[2] 张红[2] 

机构地区：[1]内蒙古林业总医院眼科,内蒙古牙克石022150 [2]哈尔滨医科大学附属第一医院眼科三病房

出　　处：《中华临床医师杂志（电子版）》2011年第15期4438-4444,共7页Chinese Journal of Clinicians(Electronic Edition)

基　　金：2008年度黑龙江省留学回国人员科技项目择优资助基金(200923);哈尔滨市青年科学研究基金留学回国人员基金(2005AFQXJ070);黑龙江省教育厅科学技术研究项目(11511155);黑龙江省首届研究生创新科研资金(YJSCX2005-302HLJ)

摘　　要：目的研究芒果苷对过氧化氢诱导的晶状体氧化损伤的保护作用,并与维生素C进行比较。方法 MTT法检测各组药物对人晶状体上皮细胞的增殖与损伤的影响;体外培养Wistar大鼠晶状体,直接观察各组药物对晶状体混浊程度的影响;并对晶状体超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)进行测定。结果 100μmol/L的过氧化氢可以诱导晶状体上皮细胞的氧化损伤和晶状体混浊(P<0.01);12.5μmol/L、25μmol/L的芒果苷可以促进HLB3细胞的生长增殖,并可抑制过氧化氢诱导的晶状体混浊,升高SOD、GSH,降低MDA,而且其作用强于100μmol/L,200μmol/L的维生素C,差异有统计学意义(P<0.01)。结论低浓度的芒果苷可以促进晶状体上皮细胞生长,对抗过氧化氢造成的氧化损伤,且抗氧化能力强于维生素C,是有效的抗氧化剂。Objective The experiment aimed at comparing the protective effects of mangiferin and vitamin C on the lens against hydrogen peroxide.Methods MTT test was used to detect the protective effects on HLB3 cells in different groups.The turbidity degree of cultured rat lens was observed in different groups.SOD,MDA and GSH were examined in different groups.Results Hydrogen peroxide(100 μmol/L) can affect the growth of HLB3 cells(P<0.01).Mangiferin(12.5 μmol/L,25 μmol/L) could promote the cell proliferation and preserve the lens from oxidation of hydrogen peroxide,SOD and GSH were increased and MDA was decreased by mangiferin at the concentration mentioned above,while 5 μmol/L mangiferin and 50 μmol/L vitamin C had no effect.Conclusions Mangiferin of low concentration could promote the growth of lens epithelial cell and protect the lens epithelial cells from the damage of hydrogen peroxide.Mangiferin has stronger antioxidative effect when compared with vitamin C.Maniferin may be a putative antioxidative agent in the treatment of cataract.

关 键 词：晶体 白内障 上皮细胞 过氧化氢 芒果苷 

分 类 号：R285[医药卫生—中药学]