冠心病合并糖尿病患者外周血Th1/Th2细胞水平的研究  

Changes of Th1/Th2 cell ratio in the peripheral blood of patients with both coronary heart disease and diabetes

作　　者：李晓燕[1] 张华丽[1] 韩淑芳[1] 康玲[1] 耿雪[1] 

机构地区：[1]济南军区总医院心内科,250031

出　　处：《中华临床医师杂志（电子版）》2011年第17期4976-4979,共4页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的通过对冠心病合并糖尿病患者外周血中Th1/Th2细胞水平变化的研究,推测Th1/Th2细胞失衡在糖尿病患者动脉硬化过程中的作用。方法选取研究对象94例(冠心病患者32例,冠心病合并糖尿病患者36例,健康对照者26例),提取外周血单个核细胞(PBMC),流式细胞术检测Th1/Th2细胞水平,分析研究对象外周血中Th1/Th2的变化。结果冠心病组Th1细胞水平较对照组明显升高,差异具有统计学意义(P<0.01);冠心病组Th2细胞水平较对照组明显降低,差异具有统计学意义(P<0.05)。冠心病合并糖尿病组患者Th1细胞水平较冠心病组和对照组明显升高,差异具有统计学意义(P<0.01);冠心病合并糖尿病组患者Th2细胞水平较冠心病组和对照组明显降低,差异具有统计学意义(P<0.05)。结论冠心病合并糖尿病患者比冠心病患者体内存在更严重的Th1/Th2失衡,合并更严重的免疫炎症反应。Objective To investigate the changes of Th1/Th2 cell ratio in the peripheral blood of patients with both coronary heart disease and diabetes,to speculate that the role what the imbalance of Th1/Th2 cells play in atherosclerosis.Methods To extract the peripheral blood mononuclear cells(PBMC)of 94 cases[32 patients with CHD,36 patients with both coronary heart disease and diabetes mellitus(CHD+DM),26 healthy controls],the Th1/Th2 cell ratio were tested by flow cytometry.Finally,we analyzed the changes of Th1/Th2 cell ratio.Results Compared with control group,the level of Th1 cells of CHD group was significantly higher,and there was significant difference(P<0.01);the level of Th2 cells was lower than the control group patients,there was significant difference(P<0.05).Compared with the CHD group and control group,the level of Th1 cells of the CHD+DM group was significantly higher(P<0.01).The difference in levels of Th2 cells between the control,CHD and CHD+DM groups was also significant(P<0.05).Conclusions Compared with patients with normal blood glucose,diabetic patients with coronary heart disease had a more serious Th1/Th2 imbalance and combined more severe immune and inflammatory responses.

关 键 词：冠心病 糖尿病 TH1细胞 TH2细胞 流式细胞术 

分 类 号：R541.4[医药卫生—心血管疾病] R587.1[医药卫生—内科学]