冠心病合并糖尿病患者外周血内皮前体细胞的研究  

The study on endothelial progenitor cells in peripheral blood of patients with coronary heart disease and diabetes mellitus

作　　者：李晓燕[1] 康玲[1] 韩淑芳[1] 高玉琪[1] 张华丽[1] 耿雪[1] 

机构地区：[1]济南军区总医院心内科,250031

出　　处：《中华临床医师杂志（电子版）》2011年第18期5346-5349,共4页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的通过对冠心病(CHD)合并糖尿病(DM)患者动脉硬化程度和外周血CD34与VEGFR-2共同标记的血管内皮前体细胞(CD34+/VEGFR-2+EPCs)水平的测定,探讨高血糖促进动脉硬化进展的作用机制。方法选取确诊为CHD患者41例和CHD合并DM患者44例,抽取外周血行流式细胞术检测CD34+/VEGFR-2+EPCs水平,并对CHD合并DM的患者行糖化血红蛋白(HbA1c)检测。结果 (1)随着冠状动脉病变程度的加重,CD34+/VEGFR-2+EPCs有下降趋势(P<0.05)。(2)EPCs等级逐渐升高,HbA1c水平逐渐下降(P<0.05)。(3)在冠状动脉不同病变水平上,CHD合并DM患者体内CD34+/VEGFR-2+EPCs水平均较CHD患者低(P<0.05)。结论高血糖易造成冠状动脉多支血管的内皮受损,EPCs水平的降低可能在高血糖加速动脉硬化过程中起重要作用。Objective To explore the mechanism in hyperglycaemia accelerating atherosclerosis development by evaluating the frequency of CD34+/VEGFR-2+EPCs in patients with coronary heart disease(CHD)and diabetes mellitus(DM).Methods 41 patients with CHD and 44 patients with CHD and DM were recruited.Blood samples were immediately disposed for direct immunofluorescence.The levels of CD34+/VEGFR-2+ EPCs were evaluated by FACS.HbA1c were tested in patients with CHD and DM.Results (1)The level of circulating CD34+/VEGFR-2+EPCs had a negative corelationship with the severy of CHD(P<0.05).(2)The level of circulating CD34+/VEGFR-2+EPCs had a negative corelationship with plasma HbA1c(P<0.05).(3)Divided by the severity of coronary,patients with CHD and DM had a lower levels of CD34+/VEGFR-2+EPCs than patients with CHD (P<0.05).Conclusions Hyperglymeamia participate in impairment of endotheli.The attenuated frequency of circulating EPCs may take part in accelerating atherosclerosis development.

关 键 词：冠心病 糖尿病 内皮 血管 前体细胞 血红蛋白A 糖基化 

分 类 号：R587.2[医药卫生—内分泌] R541.4[医药卫生—内科学]