三种人胚胎许旺细胞培养方法的比较  

Comparison of different culture methods on human embryonic schwann cells

作　　者：王洪美[1] 胡琴[2] 姜晓荣[1] 肖娟[1] 任玉水[1] 陈琳[1] 黄红云[1] 

机构地区：[1]北京市虹天济神经科学研究院北京康复中心神经外科,100144 [2]北京大学医学部人体解剖与组织胚胎学系

出　　处：《中华临床医师杂志（电子版）》2011年第19期5599-5603,共5页Chinese Journal of Clinicians(Electronic Edition)

基　　金：首都医学发展科研基金(2009-3229)

摘　　要：目的比较三种不同培养方法对许旺细胞纯度影响。方法应用三种不同的培养方法对10份人胚胎样本的坐骨神经分别采用植块培养法、差速贴壁法、分次消化法进行培养观察。并采用S100免疫荧光化学方法鉴定许旺细胞,Hoechst染色标记细胞核,统计许旺细胞纯度。结果三种培养方法其获得的细胞纯度分别为:分次消化培养法为90%,差速贴壁法为75%,植块培养法为67%,分次消化培养法获得的细胞纯度明显高于其他两组(P<0.05)。结论分次消化培养法较差速贴壁法、植块培养法更易获得高纯度许旺细胞。Objective To established a high yield and purity primary culture method of Schwann cells derived from human embryo.Methods 10 sciatic nerve derived from human fetuses were obtained, sliced, digested in trypsin and made into monoplast suspension. Cells were cultured respectively by explant culture method, differential adhesion method and enzyme digestion method for 7 days. Double-label immunofluorescence staining of S100 and Hoechst were obtained to calculate the purified rate of Schwann cells. Results The percentage of Schwann cells is 90% in enzyme digestion group, much higher than that in explant culture group and differential adhesion group which were 67% and 75% respectively. Conclusions The enzyme digestion method allows for more high-purity enrichment of Schwann cells than explant culture method and differential adhesion method.

关 键 词：许旺细胞 坐骨神经 S100蛋白质类 纯化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]