软骨素酶ABC增加化学去细胞异体神经修复神经缺损长度的实验研究  

Chondroitinase ABC enhanced the length of chemically extracted acellular nerve in repairing peripheral nerve defect

作　　者：于海龙[1] 彭江[2] 卢世璧[2] 项良碧[1] 张莉[2] 赵斌[2] 眭翔[2] 许文静[2] 

机构地区：[1]沈阳军区总医院骨科,110016 [2]解放军总医院全军骨科研究所

出　　处：《中华临床医师杂志（电子版）》2011年第19期5604-5607,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：辽宁省博士启动基金(20101134)

摘　　要：目的应用软骨素酶ABC去除化学去细胞异体神经中不利于神经再生的物质——硫酸软骨素蛋白多糖(CSPG),修复大鼠坐骨神经长距离缺损,评价去除CSPG对修复缺损距离的影响。方法经软骨素酶ABC处理后化学去细胞异体神经修复大鼠20mm坐骨神经缺损为实验组(A组),对照组为未经酶处理的化学去细胞异体神经移植组(B组),术后3个月,评价其修复周围神经缺损的效果。结果术后3个月,两组都出现残足情况,A组仅一只大鼠出现残足,而B组6只大鼠出现残足。据Wall自残标准评分,两组进行等级资料秩和检验,两者之间具有统计学差异(P<0.05)。术后3个月,A组10只大鼠8只出现钳夹痛觉实验阳性,而B组仅1只大鼠出现阳性反应,两组之间具有显著性差异(χ2=4.42,P<0.05)。在小腿三头肌肌张力恢复率和小腿三头肌肌湿重方面,两组比较A组均优于B组(P<0.05)。进行近端与远端吻合口HE染色A组神经纤维结构优于B组。结论经软骨素酶ABC处理的化学去细胞异体神经可以有效地增加修复周围神经缺损的长度。Objective The protein of chondroitin sulfate proteoglycan (CSPG) in chemcially extracted acellular nerve (CEAN) was to be degraded by chondroitin enzyme ABC,and the peripheral nerve large defects was to be repaired with these CEAN in order to evaluate the effect of degrading CSPG of CEAN on the length of repairing nerve defects.Methods The Wister rats which left sciatic nerve was cut for 20 mm defect were randomly divided into two groups:CEAN degraded by chondroitin enzyme ABC (A group),CEAN (B group).The result of nerve regeneration was evaluated 3 months after operation.Results Animal experiment evaluated by functional and histological assessment 3 months after operation showed that there was autotomy in both groups.There was only one rat that had autotomy foot in A group but 6 rats in B group.Assessment of autotomy was carried out using the scale described by Wall,there were significant difference between both groups (P<0.05).8 rats had positive pain reaction test in A group while as 1 rat did in B group.There was significant difference between them according to Chi-square test of four-fold table (P<0.05).The percentage of the triceps surae muscular tension and triceps surae weight in A group were better than the B group (P<0.05).Nerve fibers structure dyed by HE staining were better in A group than in B group. Conclusions CSPG can be removed from the CEAN degraded by chondroitin enzyme ABC.Chondroitinase treatment increases the effective length of acellular nerve grafts.

关 键 词：软骨素裂合酶类 周围神经 化学去细胞异体神经 神经缺损 

分 类 号：R651.3[医药卫生—外科学]