深低温冷冻对鼠异体异位气管移植体免疫调节的影响  

Effect of immunoregulation on rat cryopreserved ectopic tracheal allografts

作　　者：闫小龙[1] 韩静[2] 周勇安[1] 姜涛[1] 韩勇[1] 汪健[1] 倪云峰[1] 赵晋波[1] 李小飞[1] 

机构地区：[1]第四军医大学唐都医院胸外科,西安710038 [2]第四军医大学唐都医院眼科,西安710038

出　　处：《中华临床医师杂志（电子版）》2011年第19期5624-5629,共6页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(39970707)

摘　　要：目的研究应用深低温冷冻处理气管移植体,异体异位移植后移植体的结构变化,并观察术后CD3阳性淋巴细胞在移植体中的浸润情况,探索应用深低温冷冻来降低免疫排斥反应的可行性。方法取SD大鼠5个环气管段冷冻后作为供体气管移植体,行冷冻或未冷冻同种异体异位移植于Wister大鼠腹腔大网膜内。64只Wister大鼠按接受移植体是否在术前行深低温冷冻及术后处死时间,等分为8组,于术后1周、4周、8周、12周分别取标本行大体及病理学检查,组织学半定量测定上皮、软骨情况,免疫组织化学法检测各组CD3阳性淋巴细胞浸润的情况。结果冷冻组气管移植体管腔通畅度优于未冷冻组(P<0.01);1、4、8周两组比较,冷冻组上皮积分低于未冷冻组(P<0.01),而软骨形态结构积分较未冷冻组高(P<0.01);冷冻组软骨积分不随术后存活时间延长减低,未冷冻组上皮积分与术后存活时间成反比,且该组软骨形态结构积分与术后存活时间成反比。CD3阳性细胞浸润主要见于黏膜下层及气管环间,可见于气管软骨,冷冻组CD3阳性细胞浸润数积分低于未冷冻组,两组间差异有统计学意义(P<0.05)。结论异体异位移植后,气管移植体管腔结构保持良好,可长期存活,深低温冷冻可降低气管移植体的抗原性,减轻移植后的免疫排斥反应。Objective To investigate the effect of cryopreserved technique on the structure of trachea grafts,the infiltration cells positive for CD3,and the possibility of clinical application of cryopreserved tracheal allotransplantation without immunodepressant.Methods Each graft consisted of a 5-ring segment which was harvested from inbred SD rats.The grafts were implanted into omenta of inbred Wister rats.Sixty-four Wister rats were randomly assigned to 8 groups according to harvesting time and whether the received tracheal segments were cryopreserved or not.The grafts were harvested 1,4,8,12 weeks after operation respectively.The tracheal segments were then evaluated grossly and histologically.The condition of epithelium and cartilage were graded semiquantitatively.The infiltration cells positive for CD3 were also detected with immunohistochemistry methods.Results Cryopreserved groups got better structural integrity than the fresh groups(P<0.01).Cryopreserved groups got lower scores of the epithelium and higher scores of cartilage than fresh groups in 1,4,8 weeks(P<0.01),Scores of cartilage were significantly related to harvest time in fresh groups(P<0.01),but not in cryopreserved groups(P>0.05).CD3 positive cells were observed in submucosa,tissue between tracheal rings and tracheal cartilage.Positive grades of CD3 in cryopreserved groups were higher than fresh groups (P<0.01).Conclusions Structural integrity and long term viability of tracheal allografts can be maintained using cryopreserved method.The antigenicity of trachea allografts and immunological rejection can be reduced by cryopreservation.

关 键 词：气管 移植 冷冻 移植物排斥 大鼠 

分 类 号：R653[医药卫生—外科学]