单一检测体系内外周血白细胞凋亡相关指标Annexin-Ⅴ、PI的时相变化特征  

Phase variation features of apoptosis correlated indicator Annexin-Ⅴ,PI of peripheral blood leucocytes in single detected system

作　　者：张立新[1] 陈亚宝[1] 叶军[1] 陆桃红[1] 林梅[1] 栾正云 刘玲 李林[1] 黄俊星[2] 吴正东[2] 

机构地区：[1]江苏省泰州市人民医院(南通大学医学院附属医院)临床医学研究所,225300 [2]江苏省泰州市人民医院(南通大学医学院附属医院)肿瘤科,225300

出　　处：《中华临床医师杂志（电子版）》2011年第22期6637-6641,共5页Chinese Journal of Clinicians(Electronic Edition)

基　　金：江苏省自然科学基金(BK2010357)

摘　　要：目的综合分析外周血白细胞凋亡相关检测指标Annexin-Ⅴ、PI的时相变化特征。方法采用直接沉降-CD45/Annexin-Ⅴ/PI法在单一检测体系内同时检测体外不同时相外周血粒细胞、单核细胞、淋巴细胞凋亡,分析其凋亡特征。结果 (1)体外0.5h时,三类细胞Annexin-Ⅴ阳性率从高到低依次为单核细胞、粒细胞、淋巴细胞,其差异具有统计学意义(H=25.055,P<0.001);PI阳性率依次为粒细胞、单核细胞、淋巴细胞,其差异具有统计学意义(H=18.762,P<0.001)。(2)随着时间的延长,各类细胞的Annexin-Ⅴ阳性率均逐渐上升,特别是单核细胞、粒细胞在6h检测结果显示大多数细胞Annexin-Ⅴ阳性(>60%);PI阳性率随着时间的延长也呈升高态势,粒细胞、单核细胞PI阳性率在3h内上升缓慢,但6h时,变化增大;(3)无论0.5h,还是6h,三类细胞Annexin-Ⅴ阳性率均明显高于PI阳性率,差异具有统计学意义(P<0.001)。结论外周血粒细胞、单核细胞、淋巴细胞凋亡各有其特点,在设计外周血白细胞凋亡相关研究方案时应考虑其不同凋亡特点,以得到准确、敏感的结果。Objective To analyze phase variation features of apoptosis correlated indicator Annexin-Ⅴ,PI of peripheral blood leucocytes generally.MethodsApoptosis of leukocytes,monocytes,lymphocytes in peripheral blood were detected simultaneously by direct sagging-CD45/annexin-Ⅴ and propidium iodide(PI)staining and at different phase(half an hour,3 hours,6 hours)in vitro single detected system.Results(1)Decrement sequence about annexin-Ⅴ positive proportion of the three kind cells was monocytes,leukocytes,lymphocytes at half hour in turn,and the statistical difference was significant(H=25.055,P<0.001).Decrement sequence about PI positive proportion of the three kind cells was monocytes,leukocytes,lymphocytes at half hour in turn,and the difference was statistically significant(H=18.762,P<0.001).(2)Annexin-Ⅴpositive proportion of the three kind cells were increased gradually following prolong time in vitro,and majority of monocytes and leukocytes were annexin-Ⅴpositive response at six hours especially(>60%).PI positive proportion of the three kind cells were increased gradually following prolong time,PI positive proportion of monocytes and leukocytes were increased slowly during 3 hours,but increased sharply at 6 hours.(3)In half an hour or 6 hours,the positive proportion of Annexin-Ⅴof the three kind cells were all higher than those of PI,and the difference was statistically significant(P< 0.001).ConclusionsThere are various apoptosis features about leukocytes,monocytes and lymphocytes in peripheral blood,and the various apoptosis features have significant value on designing plan about studying apoptosis of peripheral blood leucocytes.

关 键 词：细胞凋亡 膜联蛋白质类 粒细胞 淋巴细胞 单核细胞 流式细胞术 碘化丙啶 

分 类 号：R446.1[医药卫生—诊断学]