慢性应激抑郁模型大鼠海马蛋白激酶A的变化及抗抑郁药物的干预作用  

Effects of fluoxetine and tianeptine on expression of PKA in hippocampus of chronic stress depression rats

作　　者：吴枫[1] 李黎黎[1] 孔令韬[1] 汤艳清[1] 

机构地区：[1]中国医科大学附属第一医院精神医学科,沈阳110001

出　　处：《中华临床医师杂志（电子版）》2011年第23期6970-6974,共5页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(81071099);辽宁省科技计划资助项目(2008225010-14)

摘　　要：目的研究氟西汀与噻萘普汀对慢性应激抑郁模型大鼠海马蛋白激酶A(PKA)表达的影响。方法将大鼠随机分为抑郁模型组、氟西汀组、噻萘普汀组和对照组。模型组、氟西汀组和噻萘普汀组给予21 d的应激刺激,此期间对照组正常饲养,刺激期间氟西汀组每天灌胃氟西汀(10 mg/kg),噻萘普汀组每天灌胃噻萘普汀(50 mg/kg),模型组和对照组每天灌胃等体积的生理盐水。行为学检测应用开场法和液体消耗实验。采用Western blotting法检测各组大鼠海马PKA的表达情况。结果应激后,模型组水平穿越格数、竖立次数、修饰次数、糖水消耗百分比均显著低于对照组(P<0.01)。应激后氟西汀组水平穿越格数、竖立次数、修饰次数和糖水消耗百分比均高于模型组(P<0.05)。应激后噻萘普汀组糖水消耗百分比高于模型组(P<0.05),水平穿越格数、竖立次数和修饰次数也高于模型组,但无统计学差异。在Western blotting法检测中,模型组大鼠海马PKA的表达水平显著低于对照组(P<0.01);氟西汀组大鼠海马PKA的表达水平高于模型组(P<0.01),与对照组无统计学差异(P>0.05);噻萘普汀组大鼠海马PKA的表达水平显著高于模型组(P<0.01),但仍低于对照组(P<0.01)。结论氟西汀和噻萘普汀均能逆转慢性应激抑郁模型大鼠海马PKA表达的降低。Objective To research the effects of fluoxetine and tianeptine on expression of PKA in hippocampus of chronic stress depression rats.Methods All the experimental rats were divided by random into 4 groups:Group of depression,Group of fluoxetine,Group of tianeptine and Group of control.The rats of Group of depression,Group of fluoxetine and Group of tianeptine were applied stress for 21 days,and meanwhile Group of control no stress.The rats of Group of fluoxetine were fed with fluoxetine(10 mg/kg),Group of tianeptine were fed with tianeptine(50 mg/kg),while another groups were fed with normal sodium of the same volume.The ethology examination was performed by using method of open-field and experiment of fluid consumption.The expression of PKA was detected by Western-blotting method.Results After the chronic stress,the horizontal crossing numbers,the erection times,the modification times and the percentage of sacchar-consumption of the rats of Group of depression were less than Group of control.The horizontal crossing numbers,the erection times,the modification times and the percentage of sacchar-consumption of Group of fluoxtine were more than those of Group of depression(P<0.05).The percentage of sacchar-consumption of Group of tianeptine was more than Group of depression(P<0.05).The horizontal crossing numbers,the erection times and the modification times of Group of tianeptine were more than those of Group of depression,but no significant difference.In Western-blotting method,the level of PKA in the hippocampus of Group of depression was less than that of Group of control(P<0.01);that of Group of fluoxetine was more than that of Group of depression(P<0.01) and no obvious difference with Group of control;that of Group of tianeptine was more than that of Group of depression(P<0.01) and less than Group of control(P<0.01).Conclusions Fluoxetine and tianeptine could reverse the reduction of expression of PKA in hippocampus of chronic stress depression rats.

关 键 词：应激 海马 环AMP依赖性蛋白激酶类 氟西汀 噻奈普汀 

分 类 号：R965[医药卫生—药理学]