ARDS和SIRS病人血浆TNF-α、IL-1β、IL-6及IL-10水平的变化  

Changes in plasma levels of TNF-alpha,IL-1beta, IL-6 and IL-10 in patients with acute respiratory distress syndrome or systemic inflammatory response syndrome

作　　者：张青[1] 李琦[1] 毛宝龄[1] 钱桂生[1] 陈正堂[1] 徐剑铖[1] 

机构地区：[1]第三军医大学新桥医院呼吸病研究所,重庆400037

出　　处：《感染．炎症．修复》2004年第3期92-94,共3页Infection Inflammation Repair

基　　金：国家自然科学基金重点资助项目(39730210)

摘　　要：目的:通过检测分析 ARDS 和 SIRS 病人血浆前炎症细胞因子肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、白介素6(IL-6)和抗炎细胞因子白介素10(IL-10)水平的变化,探讨这些细胞因子在全身炎症反应和急性肺损伤中的作用。方法:选择临床诊断 ARDS 病例22例和 SIRS 8例,以及正常对照10例,收集血浆,采用酶联免疫法(ELISA)检测 TNF-α、IL-1β、IL-6和 IL-10蛋白含量。结果:SIRS 病人血浆 TNF-α、IL-1β、IL-6和 IL-10含量分别为206.1±85.9 pg/ml、313.6±76.7 pg/ml、141.4±41.5 pg/ml 和259.6±54.34 pg/ml,均显著低于ARDS 病人(分别为629.3±187 pg/ml、892.4±209 pg/ml、261.1±71.3 pg/ml 和458.1±111.93 pg/ml);但SIRS 和 ARDS 病人上述细胞因子水平均显著高于正常对照组。结论:TNFα、IL-1β和 IL-6是 SIRS 和 ARDS 演变中的重要炎症细胞因子,抗炎细胞因子 IL-10的过度释放在促进炎症反应向失控发展、急性肺损伤发展成ARDS 中发挥一定作用。To investigate the inflammatory mechanisms of tumor necrosis factor α(TNFα),interleu- kin-1β(IL-1β),interleukin-6(IL-6)and interleukin-10(IL-10)in acute respiratory distress syndrome(ARDS). Methods:Enzyme-linked immunosorbent assay(ELISA)was employed to determine the plasma levels of TNFα, IL-1β,IL-6 and IL-10 in patients with ARDS(n=22)and systemic inflammatory response syndrome(SIRS,n= 8),and ten healthy controls.The values were 629.3±187 pg/ml,892.4±209 pg/ml,261.1+71.3 pg/ml and 458.1±111.93 pg/ml,respectively in patients with ARDS.They were significantly increased in patients with ARDS and SIRS as compared to that of normal controls,and the levels in patients with ARDS were higher than pa- tients with SIRS.Conclusions:Increased release of pro-inflammatory cytokines,such as TNFα,IL-1β,IL-6,and anti-in- flammatory cytokine IL-10,may be one of the underlying mechanisms for the development of ARDS and SIRS.

关 键 词：肿瘤坏死因子α 白介素1Β 白介素6 白介素10 全身炎症反应综合征 急性呼吸窘迫综合征 

分 类 号：R56[医药卫生—呼吸系统]