防疤烧伤膏对大鼠创面愈合过程中TGFβ_1基因表达的影响  

The effect of Fang Ba Shao Shang ointment on TGFβ_1 gene expression in rat skin at different stages of wound healing

作　　者：董永洪[1] 包海芬 

机构地区：[1]武警浙江省消防总队医院,杭州310011

出　　处：《感染．炎症．修复》2004年第1a2期43-45,共3页Infection Inflammation Repair

摘　　要：目的:观察防疤烧伤膏对大鼠伤口愈合过程中 TGFβ_1,mRNA 表达及蛋白含量的影响。方法:采用大鼠皮肤切除致伤模型,创面外用防疤烧伤膏,并采用碱性成纤维细胞生长因子(basic fibroblast growth,bFGF)和空白处理作为阳性和阴性对照。伤后3、7、14d 取创面组织,经10%中性福尔马林固定,石蜡包埋,切片后采用免疫组化和原位杂交的方法检测伤口组织中 TGFβ_1mRNA 和其蛋白含量的变化。结果:与空白对照组相比,防疤烧伤膏处理后的创面组织细胞内 TGFβ_1的 mRNA 和蛋白含量明显减少。结论:防疤烧伤膏在促进创面愈合的同时,能通过抑制创面组织细胞中 TGFβ_1的基因表达和蛋白含量的升高,因此,可能使伤口愈合后的瘢痕形成减少。Objective:To explore the effect of Fang Ba Shao Shang ointment on TGFβ_1 gene expression and pro- tein content in rat skin in the course of wound healing.Method:Excision wound of skin were produced in rats. Wounds were treated with Fang Ba Shao Shang ointment.Similar wounds treatment with basic fibroblast growth factor or placebo served as positive or negative controls,respectively.Wound tissues were harvested on days 3,7 and 14 after injury.The gene expression and protein content of TGF β_1 were assayed respectively with in situ hy- bridization and immunohistochemistry methods.Results:In comparison with placebo group,bFGF mRNA and pro- tein contents in tissues treated with Fang Ba Shao Shang ointment were significantly decreased at different stages of wound healing.Conclusion:The inhibitory effect of Fang Ba Shao Shang ointment on TGF_1 gene expression and protein synthesis might be able to decrease scar formation.

关 键 词：TGFβ_1基因表达 伤口愈合 防疤烧伤膏 

分 类 号：R28[医药卫生—中药学]