低温保存皮肤纤维连接蛋白和层粘连蛋白的表达  

The immunohistochemical study of fibrunectin and laminin in cryopreserved human skins

作　　者：贾晓明[1] 王翠娥[2] 吴小红[2] 李豫川[2] 曹军田[2] 甘永华[2] 蔡宏[1] 

机构地区：[1]解放军第304医院全军烧伤研究所临床部,北京100037 [2]军事医学科学院微生物流行病研究所北京,100071

出　　处：《感染．炎症．修复》2003年第4期214-216,共3页Infection Inflammation Repair

基　　金：国家自然科学基金资助(30070298)

摘　　要：目的:观察细胞外基质中纤维连接蛋白(FN)、层粘连蛋白(LN)在不同温度保存皮肤组织中的分布,以探讨其与皮肤冷冻损伤的关系。方法:用 FN、LN 抗体对5种保存条件下的皮肤作免疫组化染色,用图像分析仪测定表皮组织中的含量,并用 H.E 染色观察皮肤的组织结构变化。结果:与新鲜皮片组相比,-196℃H抗冻液中保存皮肤组织结构保存较好,FN、LN 含量变化不明显,而4℃、-20℃、-80℃保存皮肤组织结构破坏明显,含量亦明显下降。结论:皮肤低温保存冷冻损伤与细胞外基质中 FN、LN 破坏有关。Objective:To investigate the distribution of fibronectin(FN)and laminin(LN)in cryopreserved skins and its relationship with cryoinjury.Methods:Under five kids of skin cryopreservation,immunohistochemi- cal staining with antibodies to FN and LN was performed to observe the changes in FN and LN levels,and mean- while histopathological examination was carried out in skins by routine method.Result:Comparing to the normal human skin,histological examination and FN,LN level of skins did not change significantly under -196℃ in H anti-frozen liquid,while the skin structure in conditions kept in 4℃,-20℃,-80℃ were damaged at certain ex- tent,together with reduction in FN and LN levels.Conclusion:The skin cryoinjury might be associated with ex- tracellular matrix.

关 键 词：皮肤 纤维连接蛋白 层粘连蛋白 冷冻保存 

分 类 号：R31[医药卫生—基础医学]