烫伤创面再上皮化过程中表皮角质形成细胞增殖分化相关基因差异性表达研究  

Study on keratinocytes proliferation and differentiation related genes during the process of re-epithelialization

作　　者：章雄[1] 刘琰[1] 张志[1] 许伟石[1] 

机构地区：[1]上海二医科大学附属瑞金医院烧伤科,200025

出　　处：《感染．炎症．修复》2005年第3期135-137,共3页Infection Inflammation Repair

摘　　要：目的：利用基因芯片技术检测创面再上皮化过程中表皮角质形成细胞(Kerationocytes,KCs)增殖分化相关基因的表达。方法：SD大鼠24只,随机平均分成四组,水浴法造成背部45cm^2深Ⅱ度烫伤,按照基因表达检测时间随机分为4组,每组6只。伤后3、10、14d取创缘1cm皮肤标本；创面完成再上皮化日,取创面中心皮肤标本,制备KCs悬液,另取6只SD大鼠背部皮肤作为正常对照组。检测再上皮化不同阶段KCs增殖分化相关基因的差异性表达。结果：再上皮化过程中cyclin-dependent kinase 2(Cdk2)β,cell division cycle 42 homolog (Cdc42),transcriptional regulator protein(Hcngp),cyclin B(Ccnbl)等增殖相关基因上调,bromodomain contai- ning 4(Brd4),mitogen activated protein kinase 3(Mapk3),mitogen activated protein kinase 8 interacting protein (Mapk8ip)等增殖相关基因下调；完成再上皮化时,增殖、分化相关上调基因中出现protein kinase C,delta (Prkcd)、Caspase3(Casp3)等。结论：KCs在烫伤后活化,MAPK信号转导通路激活,Cdk2β、Cdc42、Hcngp、Cc- nbl等增殖促进因子基因表达上调,使细胞开始增殖；再上皮化过程中,既有增殖相关基因上调,又有下调；完成再上皮化时,Prkcd、Casp3等基因表达上调,调节KCs的增殖和分化。Objeetive:To investingate keratinocyte(KCs)proliferation and differentiation related gene expression during the process of re-epithelialization with cDNA microarray technique.Methods:24 SD rats were subject to a deep partial thickness scalding on the back,and 6 rats were used as normal controls.Tissue samples of 1cm of wound margin were collected on postburn days 3,10 and 14.At the end of re-epithelialization,tissue samples were harvested from the center of the wounds.Samples were treated with enzyme digestion,and KC suspensions were prepared,cDNA assay was performed in KC.The animal were ramdonized into 4 groups according to the time of microassay after the scald injury:3d,10d,14d,and after complete epithelidization.Results:During the process of re-epithelialization gene expression of transcript regulator protein,cyclin-dependent kinase 2(Cdk2)β, cell division cycle 42 homolog(Cdc42),transcriptional regulator protein(Hengp),cyclin B(Ccnb1)were up reg- ulated.Gene expressions of bromodomain containing 4(Brd4),mitogen activated protein kinase 3(Mapk3),mi- togen activated protein kinase(Mapk)8 interacting protein(Map8ip)were down regulated.At the end of re-epi- thelialization,protein kinase C,delta(Prkcd),Caspase 3(Casp3)were up regulated.Conclusion:KCs as well as MAPK signal transduction pathway were activated after injury.Gene expression of transcript regulator protein, Cdk2β,Cdc42,Hcngp,Ccnbl were up regulated,thereby inducing the cell proliferation.During the process of re- epithelialization,both up-regulation and down-regulation of genes were observed.At the end of re-epithelializa- tion,Prkcd and Casp3 were up regulated,thus regulating the proliferation and differention of KCs.

关 键 词：基因芯片 再上皮化 表皮角质形成细胞 增殖 分化 

分 类 号：R64[医药卫生—外科学]