SYTO/EB双重染色法测定皮肤活力  

SYTO/EB dyes for detection of skin viability

作　　者：贾晓明[1] 纪晓峰[1] H.Yang 

机构地区：[1]解放军第304医院烧伤研究所临床部,北京100037 [2]加拿大阿尔伯塔大学医学与病理系

出　　处：《感染．炎症．修复》2001年第4期209-210,共2页Infection Inflammation Repair

基　　金：全军回国人员科研启动基金(批准号97H30)

摘　　要：目的:建立一种简便、快速和灵敏的皮肤组织原位活力检测方法。方法:采用SYTO 13和溴化乙啶(EB)两种核酸染色剂对皮肤组织进行双重染色,荧光显微镜下通过颜色不同判断细胞的活力状态并计算出活力百分比。本研究利用SYTO/EB法和一种快速、灵敏的间接活力测定方法WST-1法同时对4℃保存不同时间的皮肤进行活力的检测,并将结果进行比较。结果:两种方法测定的结果均显示皮肤在一定的保存液中4℃保存后两周时活力为新鲜皮肤的30%左右,两个结果在各时相点均未有明显差异。结论:SYTO/EB测定皮肤的活力快速、灵敏且简便,并可原位观察皮肤内各种细胞的活力,不失为一种可取的皮肤活力检测方法。Objective: To develop an assay for detection of skin viability in situ. Methods: Two nucleic acid dyes, SYTO 13 and EB, were used to assess the viability of skin tissue. The viability of cells could be determined by different colors under fluorescence microscope. In this study, the recovery of skin restored at 4℃ for different periods was measured by SYTO/EB dyes and a type of indirect assessment, WST-1. Then both results werecompared. Results: The results measured by two methods showed that the viability of skin tissue was nearly 30% after two weeks storage. At any point of experiment, no marked difference was found between the two methods. Conclusion: The assessment of skin viability by SYTO/EB is quick, sensitive and convenient. At the same time, the viability of any type of cells can be observed in situ. So it can be considered as an available method for detection of skin viability.

关 键 词：SYTO 溴化乙啶 皮肤 活力 

分 类 号：R751[医药卫生—皮肤病学与性病学]