Tissue Culture Technology for Populus × euramericana Hybrids: Effect of Genotype on in Vitro Regeneration  

作　　者：XIA Xinli ZHANG Hongmei ZHANG Xingfu YIN Weilun 

机构地区：[1]College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,P.R.China [2]Institute of Genetics and Physiology,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050051,P.R.China

出　　处：《Chinese Forestry Science and Technology》2008年第4期47-52,共6页中国林业科技(英文版)

摘　　要：In order to select a suitable genotype for the studies on genetic transformation, the difference among three genotypes (DN04-18, DN04-25 and DN04-26) of Populus × euramericana (Populus deltoides × P. nigra) in terms of shoot regeneration was investigated in this paper. An efficient in vitro micropropagation method was successfully established for genotype DN04-18. The high frequency shoot regeneration (90.0%) from leaf discs was obtained in genotype DN04-18 on the modified Murashige and Skoog (mMS) medium with 30 g/L sucrose and 5.5 g/L agar (pH: 5.8) which was supplemented with 0.8 mg/L 6-benzyladenine (6-BA), while the shoot regeneration rates of DN04-25 and DN04-26 were below 40.0% even on the same medium. The optimum rooting medium was 1/2 mMS medium supplemented with 0.3-0.4 mg/L indole-3-butyric acid (IBA) for three genotypes. The results showed that genotype is a key factor for restricting shoot regeneration in establishing regeneration system of P. × euramerican hybrids. This micropropagation system of DN04-18 is expected to be suitable for the studies on gene transformation to improve drought tolerance of P. × euramerican.In order to select a suitable genotype for the studies on genetic transformation, the difference among three genotypes (DN04-18, DN04-25 and DN04-26) of Populus × euramericana (Populus deltoides × P. nigra) in terms of shoot regeneration was investigated in this paper. An efficient in vitro micropropagation method was successfully established for genotype DN04-18. The high frequency shoot regeneration (90.0%) from leaf discs was obtained in genotype DN04-18 on the modified Murashige and Skoog (mMS) medium with 30 g/L sucrose and 5.5 g/L agar (pH: 5.8) which was supplemented with 0.8 mg/L 6-benzyladenine (6-BA), while the shoot regeneration rates of DN04-25 and DN04-26 were below 40.0% even on the same medium. The optimum rooting medium was 1/2 mMS medium supplemented with 0.3-0.4 mg/L indole-3-butyric acid (IBA) for three genotypes. The results showed that genotype is a key factor for restricting shoot regeneration in establishing regeneration system of P. × euramerican hybrids. This micropropagation system of DN04-18 is expected to be suitable for the studies on gene transformation to improve drought tolerance of P. × euramerican.

关 键 词：Populus×euramericana GENOTYPE shoot regeneration tissue culture 

分 类 号：S79[农业科学—林木遗传育种]