细胞内外ifnγ和IL-4在评估儿童慢性乙型肝炎Th1/Th2应答中的价值  

extracellular and intracellular ifnγ and il-4 in evaluating th1/th2 responses in children with chronic hepatitis b

作　　者：陈怡[1] 苏东辉[2] 潘晨[1] 黄祖雄[1] 

机构地区：[1]福州市传染病医院肝病研究所,350025 [2]福建医科大学免疫学系

出　　处：《中华临床感染病杂志》2011年第1期-,共3页Chinese Journal of Clinical Infectious Diseases

基　　金：福建省青年科研课题项目，福建医科大学教授研究基金项目

摘　　要：目的 检测细胞内外ifnγ和il-4水平,评估慢性乙型肝炎(chb)和hbv携带患儿th1/th2应答状态.方法 收集2007年5月-2009年2月54例住院患儿,将其分为chb组(23例)和hbv携带组(31例),另外选取34名健康儿童作为对照.采用elisa法检测研究对象全血细胞外ifnγ和il-4,采用流式细胞术检测细胞内ifnγ和il-4.方差齐者行方差分析,不齐者行非参数kruskal-wallis检验分析.结果 elisa法检测发现,ifnγ、il-4水平和th1/th2比值在chb组、hbv携带组和健康对照组间差异无统计学意义(f=0.342、0.020和0.507,p值均＞0.05).流式细胞术检测发现细胞内ifnγ水平在chb组、hbv携带组和健康对照组分别为(7.68±4.62)、(11.71±4.36)和(13.61±6.71)μg/ml,th1/th2比值分别为0.96±0.30、1.67±0.76和2.11±1.12,差异有统计学意义(f=0.255和0.140,p＜0.05或＜0.01);il-4水平在三组间的差异无统计学意义(f=0.425,p＞0.05).结论 chb和hbv携带组患儿细胞内因子表达失衡,细胞免疫功能受到抑制.与elisa法相比,流式细胞技术能够更加真实、准确地反映th1和th2的应答状态. abstract： objective to evaluate extracellular and intracellular ifnγ and il-4 levels in assessing th1/th2 balance in children with chronic hepatitis b (chb) and asymptomatic carriers.methods fiftyfour hospitalized children including 23 chb patients and 31 asymptomatic carriers were collected from may 2007 to february 2009.thirty-four healthy children were served as control.serum ifnγ and il-4 levels were measured by enzyme-linked immunosorbent assay (elisa) and intracellular ifnγand il-4 levels were detected by flow cytometer.analysis of variance (for homogenous variance) and kruskal-wallis test (for non-homogenous variance) were performed.results the differences on extracellular ifnγ, il-4 and th1/th2 among chb, asymptomatic carriers and control groups were not statistically significant (f=0.342, 0.020 and 0.507, p ＞ 0.05); while the intracellular ifnγlevels were (7.68 ± 4.62), (11.71 Objective To evaluate extracellular and intracellular IFNγ and IL-4 levels in assessing Th1/Th2 balance in children with chronic hepatitis B (CHB) and asymptomatic carriers.Methods Fiftyfour hospitalized children including 23 CHB patients and 31 asymptomatic carriers were collected from May 2007 to February 2009.Thirty-four healthy children were served as control.Serum IFNγ and IL-4 levels were measured by enzyme-linked immunosorbent assay (ELISA) and intracellular IFNγand IL-4 levels were detected by flow cytometer.Analysis of variance (for homogenous variance) and Kruskal-Wallis test (for non-homogenous variance) were performed.Results The differences on extracellular IFNγ, IL-4 and Th1/Th2 among CHB, asymptomatic carriers and control groups were not statistically significant (F=0.342, 0.020 and 0.507, P ＞ 0.05); while the intracellular IFNγlevels were (7.68 ± 4.62), (11.71 ±4.36) and (13.61 ±6.71) μg/mL, and Th1/Th2 ratios were 0.96 ±0.30, 1.67 ±0.76 and 2.11 ± 1.12in three groups respectively (F=0.255 and 0.140, P ＜ 0.05 or ＜ 0.01).The differences in intracellular IL-4 levels among three groups were not significant (F=0.425, P ＞ 0.05).Conclusions Cytokine balance is affected in CHB children and asymptomatic carriers, and flow cytometry analysis is considered as a better method in evaluating the status of Th1/Th2 balance.

关 键 词：肝炎 乙型 慢性 儿童 干扰素γ 白细胞介素4 流式细胞技术 

分 类 号：R512.62[医药卫生—内科学]