耐药铜绿假单胞菌临床分离株发现aac(6')-Ⅰb-cr基因  

aac(6')-Ⅰ b gene in drug-resistant strains of pseudomonas aeruginosa

作　　者：林宁[1] 孙海平[1] 

机构地区：[1]江苏省淮安市第一人民医院检验科,223300

出　　处：《中华临床感染病杂志》2011年第3期-,共5页Chinese Journal of Clinical Infectious Diseases

摘　　要：目的 调查耐药铜绿假单胞菌中氨基糖苷类修饰酶基因(ame)和16s rrna甲基化酶基因的存在情况.方法 对分离自江苏省淮安市第一人民医院住院患者送检痰液和伤口分泌物样本的耐药铜绿假单胞菌共20株,采用聚合酶链反应(pcr)及序列分析研究8种氨基糖苷类修饰酶基因[aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6')-Ⅰ b、aac(6')-Ⅱ、ant(2")-Ⅰ、ant(3")-Ⅰ ant(4')-Ⅰ、aph(3')-Ⅱb]和6种16s rrna甲基化酶基因(arma、rmta、rmtb、rmtc、rmtd、npma).结果 20株铜绿假单胞菌共检出4种ame基因:aac(6')-Ⅱ8株(40.0%)、ant2"-Ⅰ 8株(40.0%)、aac(3)-Ⅱ5株(25.0%)、aac(6')-Ⅰ b 2株(10.0%),以及1种16s rrna甲基化酶基因:rmtb 1株(5.0%),其余9种基因未检出.对2株aac(6')-Ⅰ b基因pcr阳性产物进行测序,证实有1株单独携带aac(6')-Ⅰ b经典型,1株单独携带aac(6')-Ⅰ b-cr双功能酶基因.结论 在耐药铜绿假单胞菌中存在aac(6')-Ⅰ b-cr型基因,且对氨基糖苷类和喹诺酮类药物均有修饰作用. abstract： objective to investigate the distribution of aminoglycoside modifying enzyme genes (ames) and 16s rrna methylase genes in drug-resistant strains of pseudomonas aeruginosa. methods twenty strains of drug-resistant pseudomonas aeruginosa were isolated from sputum and wound secretion samples collected from the first people's hospital of huai' an in jiangsu province. eight ames [aac(3)-Ⅰ , aac(3)-Ⅱ, aac(6')-Ⅰb,aac(6')-Ⅱ, ant{2")-Ⅰ ,ant(3")-Ⅰ , ant(4')-Ⅰ , aph(3')-Ⅱb] and 6 16s rrna methylase genes (arma, rmta, rmtb, rmtc, rmtd, npma) were analyzed by pcr and verified by dna sequencing. results out of 20 strains of pseudomonas aeruginosa, aac(6')-Ⅱ was positive in 8 strains (40.0% ) , ant2"- Ⅰ in 8 strains (40.0% ) , aac(3)-Ⅱ in 5 strains (25.0% ) , aac(6')- Ⅰ b in 2 strains (10.0% ) and rmtb in 1 strains (5.0% ) , respectively. the rest 9 genes were not detected. among 2 strains harboring aac(6')- Ⅰ b, dna sequencing confirmed that 1 was aac(6')- Ⅰ b (tObjective To investigate the distribution of aminoglycoside modifying enzyme genes (AMEs) and 16S rRNA methylase genes in drug-resistant strains of Pseudomonas aeruginosa. Methods Twenty strains of drug-resistant Pseudomonas aeruginosa were isolated from sputum and wound secretion samples collected from the First People's Hospital of Huai' an in Jiangsu province. Eight AMEs [aac(3)-Ⅰ , aac(3)-Ⅱ, aac(6')-Ⅰb,aac(6')-Ⅱ, ant{2")-Ⅰ ,ant(3")-Ⅰ , ant(4')-Ⅰ , aph(3')-Ⅱb] and 6 16S rRNA methylase genes (armA, rmtA, rmtB, rmtC, rmtD, npmA) were analyzed by PCR and verified by DNA sequencing. Results Out of 20 strains of Pseudomonas aeruginosa, aac(6')-Ⅱ was positive in 8 strains (40.0% ) , ant2"- Ⅰ in 8 strains (40.0% ) , aac(3)-Ⅱ in 5 strains (25.0% ) , aac(6')- Ⅰ b in 2 strains (10.0% ) and rmtB in 1 strains (5.0% ) , respectively. The rest 9 genes were not detected. Among 2 strains harboring aac(6')- Ⅰ b, DNA sequencing confirmed that 1 was aac(6')- Ⅰ b (the clssical type) and another was aac(6')- Ⅰ b-cr. Conclusion Gene aac(6')- Ⅰ b-cr exists in drug-resistant Pseudomonas aeruginosa, and it has modifying effect on both aminoglycosides and quinolones.

关 键 词：铜绿假单胞菌 氨基糖苷类修饰酶基因 16s rrna甲基化酶基因 

分 类 号：R446.5[医药卫生—诊断学]