流式细胞术在真菌药物敏感性试验中的临床应用  

flow cytometry method for antifungal susceptibility test and its clinical application

作　　者：徐瑞龙[1] 朱以军[1] 卜黎红[1] 许健波[1] 潘芸[1] 单小云[1] 郑昭瓃[1] 吴俊庆 王惠姣[3] 

机构地区：[1]321000,浙江省金华市中心医院检验科 [2]金华市中医院检验科 [3]金华市人民医院检验科

出　　处：《中华临床感染病杂志》2011年第6期-,共5页Chinese Journal of Clinical Infectious Diseases

基　　金：金华市科技局基金资助项目

摘　　要：目的 建立应用流式细胞术进行真菌快速药敏试验的方法并探讨其临床应用价值.方法 对流式细胞术检测真菌药敏的实验条件进行优化,应用该法检测230株假丝酵母菌对氟胞嘧啶、氟康唑、伊曲康唑、两性霉素b的敏感性,并将其检测结果与m27-a2的标准化方法结果进行比较.结果 流式细胞术所检测的mic值与m27-a2标准化方法的检测mic结果一致率为85.0％～100％.结论 流式细胞术与标准方法的检测结果具有很好的一致性,并具有快速、准确的特点. abstract： objective to develop a rapid detection method of flow cytometry for antifungal susceptibility testing.methods the experiment conditions of flow cytometry were optimized and the minimum inhibitory concentrations (m1cs) of 230 strains of candida spp to flucytosin,fluconazol,itraconazole,amphotericin b were detected.the mic results obtained by flow cytometry were compared with m27-a2 reference assay.results the agreement of mic results between flow cytometry and m27-a2method was 85.0％ - 100％.conclusion flow cytometry can be used as a rapid and reliable method for antifungal susceptibility testing.Objective To develop a rapid detection method of flow cytometry for antifungal susceptibility testing.Methods The experiment conditions of flow cytometry were optimized and the minimum inhibitory concentrations (M1Cs) of 230 strains of Candida spp to flucytosin,fluconazol,itraconazole,amphotericin B were detected.The MIC results obtained by flow cytometry were compared with M27-A2 reference assay.Results The agreement of MIC results between flow cytometry and M27-A2method was 85.0％ - 100％.Conclusion Flow cytometry can be used as a rapid and reliable method for antifungal susceptibility testing.

关 键 词：流式细胞术 真菌 微生物敏感性试验 

分 类 号：R446.5[医药卫生—诊断学]