离体施万细胞实验性高血糖损伤机制的实验研究  

Experimental study on mechanism for effect of high glucose on proliferative ability of extracorporeal Schwann cells

作　　者：吴波水[1] 王文健[2] 陈伟华[2] 应键[2] 汪洋[3] 席静[3] 蔡晓霖 

机构地区：[1]复旦大学附属华山医院神经内科,上海200040 [2]复旦大学中西医结合研究所,上海200040 [3]复旦大学医学院微生物教研组,上海200032 [4]福建省晋江市医院中医科,晋江362200

出　　处：《神经病学与神经康复学杂志》2011年第3期137-140,共4页Journal of Neurology and Neurorehabilitation

基　　金：上海市科委重点项目资金资助(编号:014319321)

摘　　要：目的同步观察高血糖对离体施万细胞增殖能力和神经生长因子(NGF)合成水平的影响,探讨周围神经再生时糖尿病性损伤的可能机制。方法采用Brockes改良法从新生Wistar乳鼠坐骨神经组织分离纯化施万细胞。采用3H-TdR掺入法检测离体施万细胞的增殖能力,用ELISA法测定离体施万细胞NGF的合成水平。结果经ABC法染色计数,抗S100免疫组化染色着色细胞的阳性率为(92±2)%,表明采用该法鉴定显示着色细胞为表达特异性抗原S100的施万细胞。高血糖组与对照组比较,细胞增殖能力和NGF合成显著受到抑制(P<0.01)。结论离体施万细胞实验性高血糖损伤所致的增殖能力下降可能与高血糖状态下施万细胞的NGF合成减少密切相关。Objective By synchronous measurement of the proliferative ability of extracorporeal Schwann cells as well as never growth factor(NGF) synthetic level and to explore the possible mechanism of high glucose on peripheral nerve regeneration.Methods Using brocks modification,Schwann cells were separated from the sciatic nerves of a newborn sucking Wister rat and purified.3H-TdR incorporative method was adopted to assay the proliferative activity of extracorporeal Schwann cells.ELISA method was used to measure the NGF level of extracorporeal Schwann cells.Results Count by the ABC staining,the positive rate of antigen-S100 immunohistochemical staining cells amounted to(92±2)%.It showed that the staining cells that were indentified by above mentioned method were Schwann ones which expressed specific antigen S100.Compared with the control group,the cell proliferative ability and NGF synthesis in the high glucose group were inhibited greatly.Conclusion The inhibitory effect on the proliferative ability of extracorporeal Schwann cells caused by high glucose is closely connected with the decrement of NGF synthesis.

关 键 词：周围神经再生 施万细胞 神经生长因子 高血糖 

分 类 号：R587.2[医药卫生—内分泌]