高频正弦波振动对吊尾大鼠脊髓c-fos蛋白表达变化的改善作用  

ameliorated effects of high frequency sinusoidal vibration on c-fos expression of rat's spinal cord during tail suspension

作　　者：杨威[1] 王晓晖[1] 张浩[1] 樊小力[2] 

机构地区：[1]山西医科大学生理学教研室,太原030001 [2]西安交通大学医学院生理学教研室,西安710061

出　　处：《中华航空航天医学杂志》2012年第1期24-28,84,共6页Chinese Journal of Aerospace Medicine

基　　金：国家自然科学基金资助项目

摘　　要：目的 观察高频正弦波振动对吊尾大鼠脊髓c-fos蛋白表达的影响. 方法 选取sprague-dawley雌性大鼠30只,按随机数字表法分为3组:模拟失重t4 d组、模拟失重+高频振动14d组及正常对照组,每组各10只动物.采用大鼠尾部悬吊法建立模拟失重模型,用免疫组织化学技术观察吊尾大鼠脊髓l5-6节段c-fos蛋白的表达. 结果 正常对照组大鼠,大多数c-fos阳性神经元位于脊髓Ⅳ和Ⅵ～Ⅶ板层.模拟失重14d后,脊髓Ⅳ、Ⅵ和Ⅶ板层内的阳性神经元数量及Ⅵ～Ⅶ板层内阳性神经元总数均明显多于正常对照组(p＜0.05)和模拟失重+高频振动组(p＜0.05).而模拟失重+高频振动组大鼠脊髓阳性神经元的总数及板层分布模式与正常对照组大鼠无统计学差异. 结论 高频正弦波振动对模拟失重状态下大鼠脊髓神经元c-fos蛋白表达的改变具有明显的改善作用.模拟失重后肌梭传人通路中脊髓神经元活动的增强可能与肌梭传人冲动减少有关.objective to investigate whether high frequency sinusoidal vibration (hfv) influences c-fos immunoreactivity in the spinal cord after an exposure of simulated weightlessness and to study the mechanism of alteration in neuronal activity of the spindle cord in muscle spindle afferent pathways.methods thirty sprague dawley female rats were randomly assigned to 14-day tailsuspension group,14-day tail suspension plus hfv group and control group.each group had 10 rats.weightlessness was simulated by tail suspension of rats.the changes in the c-fos expression of spinal cord was detected by using immunohistochemistry technique. results the results showed that c-fos immunoreactive neurons in control group were mainly concentrated in lamina Ⅳ-Ⅶ of the ipsilateral spinal cord.by 14 days of tail-suspension,both the total number of c-fos immunoreactive neurons and the pattern of their lamellar distribution showed significant change. the number of c-fos immunoreactive neurons in lamina Ⅳ,Ⅵ and Ⅶ and the total number of labeled neurons in lamina Ⅳ-Ⅶ were significantly increased when compared with those of control rats (p＜0.05).however,after 14-day tail-suspension plus hfv,the total number of c-fos immunoreactive neurons and the pattern of their lamellar distribution did not show substantial changes as compared with those of the control group (p＞0.05).the number of c-fos immunoreactive neurons in lamina Ⅳ,Ⅵ and Ⅶ and the total number of labeled neurons were significantly decreased when compared with those of tail-suspension rats (p＜0.05). conclusions our findings show that hfv plays an obvious resistant role against the increase of c fos expression of spinal cord under the condition of simulated weightlessness.it is suggested that the significant increase in neuronal excitation of the spinal cord in muscle spindle afferent pathways under the condition of simulated weightlessness is closely related to the decrease in the afferent message from muscle spindles.

关 键 词：失重模拟 脊髓 肌梭 基因 免疫组织化学 

分 类 号：R85[医药卫生—航空、航天与航海医学]