T-2毒素对软骨细胞整合素表达的影响及硒的拮抗作用  

Effect of T-2 toxin on integrins expression and antagonistic role of selenium

作　　者：王加丽[1] 罗明秀[1] 李锦[1] 陈静红[1] 付强[1] 王伟[1] 张增铁[1] 曹峻岭[1] 

机构地区：[1]西安交通大学环境与疾病相关基因教育部重点实验室暨卫生部微量元素与地方病重点实验室,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2012年第3期271-275,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30872187;31070725)~~

摘　　要：目的研究T-2毒素和硒对软骨细胞整合素mRNA表达的影响,探讨T-2毒素和硒与大骨节病发病的关系。方法体外单层培养C-28/I2软骨细胞,用MTT法测定细胞在不同浓度T-2毒素和不同作用时间下的增殖活性,通过Real-time PCR法检测软骨细胞在加T-2毒素或补硒后整合素α1、α2、α3、β1、β3、β5等10种亚基的表达情况。结果 T-2毒素可抑制C-28/I2软骨细胞系的增殖活性,在T-2毒素作用下软骨细胞系中整合素的表达谱有显著变化,其中显著上调的整合素亚基有αv和β1,下调的亚基有α2、α5和β5,加硒可在一定程度上拮抗这种改变。结论 T-2毒素对软骨细胞增殖有明显的抑制作用,而硒能通过拮抗整合素的表达而发挥对软骨细胞的保护作用。Objective To study the effects of T-2 toxin and selenium on integrins expression to explore the relationship of T-2 toxin and selenium with the etiology of Kashin-Beck disease.Methods We used MTT assay to assess the proliferation of chondrocytes C-28/I2 treated with different concentrations of T-2 toxin and select the optimal time and concentration of T-2 toxin.Meanwhile,Real-time PCR was used to analyze the expression of integrins treated wih T-2 toxin or selenium.Results T-2 toxin could inhibit the proliferation of chondrocytes C-28/I2.Integrins profile changed with the addition of T-2 toxin;the significantly upregulated integrins were αv and β1,while the downregulated integrins were α2,α5 and β5.Moreover,selenium could partly block the integrins changes.Conclusion T-2 toxin could obviously inhibit the growth of chondrocytes while selenium could protect chondrocytes by antagonizing the expression of integrins.

关 键 词：大骨节病 T-2毒素 软骨细胞 整合素 硒 

分 类 号：R96[医药卫生—药理学]