宁夏回族高血压患者MSA2756G、CYP2C9*3多态性的研究  

CYP2C9*3 and MSA2756G gene polymorphisms in hypertensive patients of Ningxia Hui nationalities

作　　者：李居怡 邹吉利 王健[2] 刘新国 陈永刚 白丹[3] 王雄 吴金虎 

机构地区：[1]武汉市第三医院药学部,湖北武汉430060 [2]宁夏回族自治区人民医院药剂科,宁夏银川750021 [3]郑州市第七人民医院社区医学与预防保健科,河南郑州450006

出　　处：《西安交通大学学报（医学版）》2012年第5期617-621,625,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：宁夏自然科学基金资助项目(No.NZ07109);宁夏卫生厅重点科研课题(No.W200707)~~

摘　　要：目的观察高血压患者致病基因MSA2756G和药物代谢酶相关基因CYP2C9*3多态性位点在宁夏回族高血压患者中的分布及其与高血压的关系。方法通过扩增引进限制性酶切位点(ACRS)和聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术对高血压患者进行基因型分析,利用χ2和t检验分析以上两个多态性位点的各基因型与宁夏回族高血压的相关性。结果①宁夏回族人群MSA2756G位点等位基因G在对照组中的频率为10.25%,而在高血压组中的频率为14.04%,两组等位基因G的频率分布差异无统计学意义(P>0.05);在男性中,等位基因G在高血压组(8.79%)与对照组(11.50%)中的分布差异无统计学意义(P>0.05);在女性中,等位基因G在高血压组(19.54%)与对照组(9.00%)中的分布差异有统计学意义(P<0.05);在高血压组,等位基因G在男性、女性中的频率各为8.79%、19.54%,差异有统计学意义(P<0.05)。②宁夏回族人群CYP2C9*3位点等位基因C在对照组中的频率为3.00%,而在高血压组中的频率为3.37%,两组等位基因C的频率分布差异无统计学意义(P>0.05);在男性中,等位基因C在高血压组(4.40%)与对照组(3.50%)中的分布差异无统计学意义(P>0.05);在女性中,等位基因C在高血压组(2.30%)与对照组(2.50%)中的分布差异无统计学意义(P>0.05)。结论 MSA2756G等位基因G是宁夏回族女性患高血压的危险因子,而与男性无关。Objective To investigate the distribution of CYP2C9*3 and MSA2756G related to disease gene and drugs therapy in hypertensive patients of Ningxia Hui nationalities.Methods We analyzed genotypes by amplification-created restriction sites(ACRS) and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in hypertensive patients so as to explore the relation of the genotypes with hypertension in the Ningxia Hui population by χ2 and t test.Results ① The frequency of allele G was 10.25% in control group and 14.04% in hypertension group,without significant difference between the two groups(P>0.05).In the males,the frequency of allele G was 11.50% in control group and 8.79% in hypertension group,without significant difference between the two groups(P>0.05).In the females,the frequency of allele G was 9.00% in control group and 19.54% in hypertension group,with a significant difference between the two groups(P<0.05).In hypertension group,the frequency of allele G was 8.79% in the male patients and 19.54% in the female patients, with a significant difference between the males and females(P<0.05).② The frequency of allele C was 3.00% in control group and 3.37% in hypertension group,with no significant difference between the two groups(P>0.05).In the males,the frequency of allele C was 3.50% in control group and 4.40% in hypertension group,with no significant difference between the two groups(P>0.05).In the females,the frequency of allele C was 2.50% in control group and 2.30% in hypertension group,without significant difference between the two groups(P>0.05). Conclusion The allele G of MSA2756G is a risk factor for hypertension in female hypertensive patients of Ningxia Hui nationalities,but it is not related to the men.

关 键 词：高血压 CYP2C9 甲硫氨酸合成酶 多态性 宁夏回族 

分 类 号：R54[医药卫生—心血管疾病]