日本鳗鲡及其僵鳗消化酶活性的比较研究  

Comparison of Digestive Enzyme Activities in Normal and Growth Retarded Japanese Eels(Anguilla japonics)

作　　者：黄永春[1] 

机构地区：[1]集美大学水产学院,厦门361021

出　　处：《动物营养学报》2012年第5期977-982,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：李尚大集美大学学科建设基金(ZC2010001)

摘　　要：本试验旨在比较不同反应温度下日本鳗鲡(Anguilla japonics)及其僵鳗消化酶活性的差异。试验选取正常生长的日本鳗鲡(126.4～140.2 g/尾)以及生长缓慢的僵鳗(3.5～8.6 g/尾)各20尾,取肝胰脏、胃、肠,分别在5、15、25、30、35、40、45和55℃反应温度下测定肝胰脏、胃、肠中蛋白酶、淀粉酶、脂肪酶活性。结果表明:正常鳗和僵鳗肝胰脏、胃、肠蛋白酶活性均在45℃时达到最高值,且蛋白酶活性(除5、15、55℃时的僵鳗蛋白酶活性外)均表现为肠>胃>肝胰脏;僵鳗肝胰脏、肠、胃蛋白酶活性分别为正常鳗的29.5%、15.7%和25.2%(P<0.05)。正常鳗和僵鳗淀粉酶活性均在30℃时达到最高值,正常鳗淀粉酶活性(除5℃时的淀粉酶活性外)表现为肝胰脏>肠>胃,僵鳗淀粉酶活性表现为肠>肝胰脏>胃;僵鳗肝胰脏、肠、胃淀粉酶活性分别为正常鳗的42.4%、73.7%和43.8%(P<0.05)。正常鳗和僵鳗脂肪酶活性均在35℃时达到最高值,正常鳗脂肪酶活性表现为肝胰脏>胃>肠,僵鳗脂肪酶活性(除35℃时的脂肪酶活性外)表现为肝胰脏>肠>胃;僵鳗肝胰脏、肠、胃脂肪酶活性分别为正常鳗的41.5%、45.6%和23.2%(P<0.05)。由此可见,日本鳗鲡僵鳗肝胰脏、胃、肠中蛋白酶、淀粉酶及脂肪酶活性均显著低于正常鳗,从而直接影响其生长发育。This experiment was conducted to study the effects of temperatures on the activities of digestive enzymes in Japanese eels Anguilla japonics(n=20) and growth retarded eels(n=20).The weight of the normal eels was from 126.4 to 140.2 g per eel,and that of growth retarded eels was from 3.5 to 8.6 g per eel.The activities of protease,amylase,and lipase in the normal eels and growth retarded eels under the condition of different temperatures(5,10,15,25,30,35,40,45 and 55 ℃) were studied.The results showed as follows: with the increasing of temperature,the digestive enzyme activities were increased at first,and then decreased.The optimum temperature of the protease in hepatopancreas,intestine and stomach was 45 ℃.In the same experimental temperature,the order of protease activity was intestine>stomach>hepatopancreas except growth retarded eels at 5,15 and 55 ℃.The protease activity in hepatopancreas,intestine and stomach of growth retarded eels was only 29.5%,15.7% and 25.2% of that of the normal eels,respectively(P<0.05).The optimum temperature of amylase activity was 30 ℃,and the order of amylase activity of the normal eels was hepatopancreas>intestine>stomach in experimental temperatures except at 5 ℃,but the order of amylase activity of growth retarded eels was intestine>hepatopancreas>stomach in experimental temperatures.The amylase activity in hepatopancreas,intestine and stomach of growth retarded eels was only 42.4%,73.7% and 43.8% of that of the normal eels,respectively(P<0.05).The optimum temperature of lipase activity was 35 ℃,and the order of lipase activity of the normal eels was hepatopancreas>stomach>intestine in experimental temperatures,but the order of lipase activity of growth retarded eels was hepatopancreas>intestine>stomach in experimental temperatures except at 35 ℃;the lipase activity in hepatopancreas,intestine and stomach of growth retarded eels was only 41.5%,45.6% and 23.2% of that of the normal eels,respectively(P<0.05).These results indicate that the activities of protease,am

关 键 词：温度 日本鳗鲡 僵鳗 消化酶活性 

分 类 号：S91[农业科学—水产科学]