维生素、矿物质与能量蛋白质水平对浙东白鹅母鹅繁殖性能、血液生殖激素浓度及生殖轴相关基因mRNA相对表达量的影响  

Effects of Vitamin,Mineral,and Energy and Protein Levels on Reproductive Performance,Blood Reproductive Hormone Concentrations and Related Gene mRNA Relative Expression in Reproductive Axis of Female Zhedong White Geese

作　　者：赵鑫[1,2] 邵涛[2] 王亚琴 罗锦标 陈维虎 孙红霞 周卫东[1] 

机构地区：[1]浙江省农业科学院畜牧兽医研究所,杭州310021 [2]南京农业大学动物科技学院,南京210095 [3]浙江省宁波市畜牧兽医局,宁波315012 [4]浙江省象山县畜牧兽医总站,象山315700

出　　处：《动物营养学报》2012年第6期1110-1118,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：浙江省宁波市农业科研攻关项目(2009C10024)

摘　　要：本试验通过在饲粮中添加维生素与矿物质、调整饲粮能量蛋白质水平,旨在研究其对浙东白鹅母鹅繁殖性能、血液生殖激素浓度和生殖轴相关基因mRNA相对表达量的影响。选择138只月龄相近的浙东白鹅种母鹅,按体重相近原则分为3组,分别饲喂不同的饲粮,试验期150 d,测定繁殖性能(平均产蛋数、平均蛋重、受精率和孵化率)、血液生殖激素[卵泡刺激素(FSH)、促黄体生成素(LH)、孕酮(P4)、雌二醇(E2)、催乳素(PRL)]浓度和生殖轴相关基因[促性腺激素释放激素(GnRH)、卵泡刺激素-β(FSHβ)、雌激素受体1(ESR1)、雌激素受体2(ESR2)、卵泡刺激素受体(FSHR)、催乳素(PRL)、催乳素受体(PRLR)]mRNA相对表达量的变化。结果表明:1)添加维生素与矿物质可显著提高浙东白鹅母鹅第1产蛋周期平均蛋重和受精率(P<0.05);提高第2产蛋周期内血液FSH和P4的浓度,降低LH浓度,改变E2、P4和PRL浓度波动(P<0.05);下调下丘脑PRLR、垂体PRL和卵巢PRLR基因的mRNA相对表达量(P<0.05),上调卵巢ESR2基因的mRNA相对表达量(P<0.05)。2)调整饲粮能量蛋白质水平可显著提高浙东白鹅母鹅第2产蛋周期平均蛋重(P<0.05);提高浙东白鹅第2产蛋周期内血液LH浓度,降低FSH浓度,改变E2和P4浓度波动(P<0.05);上调下丘脑GnRH、垂体PRL和PRLR基因的mRNA相对表达量(P<0.05),下调卵巢FSHR基因的mRNA相对表达量(P<0.05)。由此得出,添加维生素与矿物质、调整饲粮能量蛋白质水平可通过影响产蛋周期内部分血液生殖激素浓度和波动,局部调节生殖轴相关基因的mRNA相对表达量,改善浙东白鹅母鹅的繁殖性能。The effects of vitamin,mineral,and energy and protein levels on reproductive performance,blood reproductive hormone concentrations and related gene mRNA relative expression in reproductive axis of female Zhedong white goose were determined by adding vitamin and mineral,and regulating dietary energy and protein level.One hundred and thirty-eight female Zhedong white geese with the same month old were divided into 3 groups according to their body weight,which were provided 3 kinds of experimental diets.In the experimental period(150 d),the changes of reproductive performance(average egg number,average egg weight,fertility rate and hatchability rate),concentrations of blood reproductive hormones [follicle stimulating hormone(FSH),luteinizing hormone(LH),progesterone(P4),estrogen(E2) and prolactin(PRL)] and mRNA relative expression of related genes [gonadotropin releasing hormone(GnRH),follicle stimulating hormone-β(FSHβ),estrogen receptor 1(ESR1),estrogen receptor 2(ESR2),follicle stimulating hormone receptor(FSHR),PRL and prolactin receptor(PRLR) ] in reproductive axis were measured.The results showed as follows: 1) adding vitamin and mineral significantly increased average egg weight and fertility rate during the 1st laying cycle(P<0.05),increased concentrations of blood FSH and P4,decreased concentration of blood LH,changed E2,P4 and PRL pattern during the 2nd laying cycle(P<0.05),down-regulated GnRH,PRL and PRLR mRNA relative expression in the hypothalamus,pituitary and ovary,respectively,and up-regulated ESR2 mRNA relative expression in the ovary(P<0.05).2) Regulating energy and protein level significantly increased average egg weight during the 2nd laying cycle(P<0.05),increased concentration of blood LH,decreased concentration of blood FSH,changed P4 and E2 pattern during the 2nd laying cycle(P<0.05),increased GnRH,PRL and PRLR mRNA relative expression in the hypothalamus and pituitary,and decreased FSHR mRNA relative expression in the ovary(P<0.05).The results of this study indicate that adding vitamin and

关 键 词：浙东白鹅 维生素 矿物质 能量蛋白质水平 繁殖性能 激素 生殖轴 MRNA 

分 类 号：S83[农业科学—畜牧学]