外源寡糖对奶山羊粪便微生物区系的影响  

Effects of Exogenous Oligosaccharides on Fecal Microflora of Dairy Goats

作　　者：祁茹[1] 温建新[1] 程明[2] 肖宇[1] 褚永康[1] 胡静[1] 朱亚俊[1] 陈俏俏 林英庭[1] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]青岛市畜牧兽医研究所,青岛266109 [3]山东省莱阳市畜牧局,莱阳265200

出　　处：《动物营养学报》2012年第6期1165-1172,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山东省自然科学基金项目(ZR2009DM021);青岛市科技计划基础研究项目(11-2-4-5-(1)-jch)

摘　　要：本试验旨在研究不同外源寡糖对奶山羊粪便微生物区系的影响。试验采用不完全拉丁方设计,选用平均体重为(32.80±2.45)kg、安装有永久性瘤胃瘘管的崂山奶山羊6只,分为6组,试验组羊分别饲喂在基础饲粮中添加1%的甘露寡糖(M OS组)、半乳甘露寡糖(GM OS组)、寡木糖(XOS组)、低聚异麦芽糖(IMO组)和果寡糖(FOS组)的试验饲粮,对照组(CT组)羊饲喂基础饲粮。共进行4期试验,每期35 d,其中预试期14 d,正试期21 d。于正试期第1、7、14、21天晨饲前采集新鲜粪便,测定粪便中乳酸杆菌、双歧杆菌、大肠杆菌和沙门氏菌的数量。结果表明:与CT组相比,试验组乳酸杆菌数量均有所增加,其中FOS组第7天开始达到极显著水平(P<0.01);试验组双歧杆菌数量增加,其中FOS组各采样时间均达到极显著水平(P<0.01);试验组大肠杆菌数量有所降低,其中M OS组和FOS组第7天达到极显著水平(P<0.01)。本试验条件下,不同外源寡糖对增加奶山羊粪便乳酸杆菌、双歧杆菌数量,降低大肠杆菌数量的作用效果不同,其中果寡糖效果较明显。The effects of different exogenous oligosaccharides on fecal microflora of dairy goats were studied in this experiment.Six Laoshan dairy goats with an average body weight of(32.80±2.45) kg fitted with permanent rumen cannula were used,and an incomplete Latin square design was applied.Goats in experimental groups were fed a basal diet supplemented with 1% mannose-oligosaccharides(MOS group),galactomannan-oliggosaccharides(GMOS group),xylo-oligosaccharides(XOS group),isomalto-oligosaccharides(IMO group) and fructo-oligosaccharides(FOS group),respectively,and those in the control group(CT group) were fed the basal diet.The experiment consisted of 4 stages with 35 d in each stage,each of which included a 14-day pre-experimental period and a 21-day experimental period.Fresh fecal sample was collected for the analysis of numbers of Lactobacillus,Bifidobacteria,E.coli.and Salmonella on day 1,7,14 and 21 before morning feeding.The results showed as follows: compared with the control group,number of Lactobacillus of experimental groups was increased,and the number of FOS group was significantly increased since day 7(P<0.01);number of Bifidobacteria was also increased,and the number of FOS group was significantly increased throughout the whole experiment(P<0.01);while number of E.coli were decreased,and the numbers of MOS group and FOS group were significantly increased on day 7(P<0.01).The results indicate that improvements on fecal Lactobacillus and Bifidobacteria and inhibition on E.coli of different exogenous oligosaccharides are different,and fructo-oligosaccharides are considered to be more efficient.

关 键 词：奶山羊 寡糖 粪便微生物 

分 类 号：S82[农业科学—畜牧学]