叶酸对超早期断奶宫内发育迟缓仔猪肝脏结构和细胞凋亡相关基因表达的影响  

Folic Acid in Intrauterine Growth Retarded Early Weaner Piglets:Effects on Hepatic Structure and Apoptosis-Related Gene Expression

作　　者：姚英[1] 陈代文[1] 刘静波[1] 毛湘冰[1] 毛倩[1] 余冰[1] 

机构地区：[1]四川农业大学动物营养研究所,动物抗病营养四川省重点实验室,雅安625014

出　　处：《动物营养学报》2012年第2期271-279,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：教育部创新团队猪抗病营养分子机制研究项目(IRTO555-5)

摘　　要：本试验旨在研究饲粮添加不同水平叶酸对超早期断奶宫内发育迟缓(IUGR)仔猪肝脏结构和细胞凋亡相关基因表达的影响。选取24头14日龄断奶、平均体重(2.79±0.34)kg的"杜×长×大"三元杂交仔公猪,随机分为3个处理,分别饲喂在基础饲粮中添加0、5和10 mg/kg叶酸的试验饲粮,每个处理8个重复,每个重复1头猪。试验期21 d。结果表明:1)饲粮添加叶酸对35日龄仔猪血清葡萄糖浓度无显著影响(P>0.05),添加5和10 mg/kg叶酸分别显著(P<0.05)和极显著(P<0.01)降低了血清甘油三酯浓度。2)饲粮添加5 mg/kg叶酸能显著降低肝细胞直径(P<0.05)。3)饲粮添加5 mg/kg叶酸显著提高了肝脏B细胞淋巴瘤蛋白2(Bcl-2)的基因表达量(P<0.05),极显著降低了Bcl-2相关X蛋白(Bax)和促凋亡相关基因肿瘤蛋白53(p53)的基因表达量(P<0.01);饲粮添加10 mg/kg叶酸,Bax和p53的基因表达量分别极显著(P<0.01)和显著(P<0.05)地降低了。4)饲粮添加叶酸对肝脏毛细血管扩张性共济失调症突变基因(ATM)、脱嘌呤嘧啶核酸内切酶1(APE-1)基因和一碳单位代谢关键酶编码基因的表达无显著影响(P>0.05)。结果提示,饲粮补充一定水平的叶酸有助于改善早期断奶IUGR仔猪35日龄时肝脏结构和细胞凋亡相关基因Bcl-2、Bax和p53的表达;本试验条件下,饲粮添加5 mg/kg叶酸效果较好。This experiment was conducted to study the effects of dietary folic acid level on hepatic structure and apoptosis-related gene expression in early weaner piglets suffered intrauterine growth retardation(IUGR).Twenty four crossbred(Duroc×Landrace×Yorkshire) male piglets,weaned at 14 days of age,with an average body weight of(2.79±0.34) kg were randomly assigned into 3 groups with 8 replicates in each group and 1 piglet per replicate.Piglets were fed diets supplemented with 0,5 and 10 mg/kg folic acid,respectively.The experiment lasted for 21 d.The results showed as follows: 1) dietary supplementation of 5(P<0.05) and 10 mg/kg(P<0.01) folic acid significantly decreased serum triglycerides concentration of piglets at 35 days of age,but had no significant effect on serum glucose concentration(P>0.05).2) Dietary supplementation of 5 mg/kg folic acid significantly decreased hepatocyte diameter(P<0.05).3) Dietary supplementation of 5 mg/kg folic acid significantly up-regulated gene expression of Bcl-2(P<0.05),but significantly down-regulated gene expression of Bax and p53(P<0.01);however,gene expression of Bax(P<0.01) and p53(P<0.05) was significantly reduced by dietary supplementation of 10 mg/kg folic acid.4) Expression of ATM and APE-1 as well as key enzyme encoding genes involved in one-carbon metabolism was not affected by folic acid supplementation.These results indicate that supplementation of folic acid at proper level can improve hepatic structure and apoptosis-related gene(Bcl-2,Bax and p53) expression of IUGR piglets;under conditions in this experiment,the optimum level is 5 mg/kg.

关 键 词：叶酸 宫内发育迟缓 肝脏结构 细胞凋亡 一碳单位 早期断奶仔猪 

分 类 号：S816.7[农业科学—饲料科学]