重组绿豆胰蛋白酶抑制剂片段对肠癌细胞SW480迁移的影响  

Effects of Recombinant Mung Bean Trypsin Inhibitor Fragments on Migration of Colon Cancer Cell SW480

作　　者：赵亚蕊[1] 李宗伟[1] 赵超[1] 付荣[1] 王兴华[2] 李卓玉[1] 

机构地区：[1]山西大学生物技术研究所,化学生物学与分子工程教育部重点实验室,山西太原030006 [2]山西大学生命科学学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2012年第1期126-129,共4页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31040018);山西省留学基金(2010-2012);山西省国际合作项目

摘　　要：探讨重组胰蛋白酶抑制剂活性片段(LysGP33)的抗肠癌效应.大肠杆菌原核表达LysGP33活性片段,GST亲和层析柱对表达蛋白进行纯化.MTT比色和细胞划痕方法检测LysGP33活性片段对SW480细胞增殖和迁移的影响.结果表明:10μmol/L LysGP33活性片段对SW480细胞的增殖活性无明显影响,但能够有效地抑制SW480细胞的迁移,并呈现时间依赖效应.绿豆胰蛋白酶抑制剂抑制了肠癌细胞的迁移,在抗肿瘤浸润和转移中具有一定的应用前景.The aim of this study was to evaluate the effects of LysGP33 on metastasis and proliferation of human colon cancer cell SW480.Recombinant mung bean trypsin inhibitor fragments were purified by GST-affinity chromatography.The changes of cell proliferation treated with GST-LysGP33 were observed by MTT assay.The migration potential was examined by wound healing assay.The results indicated that the migration of SW480 cells was inhibited significantly when the cells were treated with 10 μmol/L GST-LysGP33.Moreover,it indicated that this alteration of migration was in the time dependent manner.On the other hand,the treated cells did not show the visible differences in proliferation with the treatment of 10 μmol/L LysGP33.Recombinant mung bean trypsin inhibitor fragments could suppress the migration of SW480,and have some prospect in anti-tumor migration and invasion.

关 键 词：绿豆胰蛋白酶抑制剂 细胞增殖 细胞迁移 

分 类 号：Q814[生物学—生物工程]