一株拟南芥镉敏感突变体的筛选及表型分析  

Isolation and Phenotype Analysis of A Cd Sensitive Mutant of Arabidopsis thaliana

作　　者：裴雁曦[1] 龚泽华[1] 李亚伟[1] 乔增杰[1] 穆遥[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2012年第2期400-404,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：回国留学人员项目(2011-007);教育部博士点(博导类)项目(20091401110004);高等学校优秀青年学术带头人支持计划(TYAL)

摘　　要：利用不同浓度梯度的CdCl2对拟南芥Col-0生态型进行处理,确定了筛选镉敏感突变体的适合浓度为90μmol/L.以镉对幼苗根长生长抑制程度为指标,对拟南芥化学诱导激活标签(XVE)T-DNA插入突变体库种子进行筛选.首先在不含有雌激素的1/2MS培养基进行大规模筛选,挑选根长抑制明显的植株,单株收取种子.在T3代复筛过程中用含有浓度为90μmol/L CdCl2的1/2MS固体培养基筛选到一株敏感突变体csr-2.并进行了雌二醇诱导过表达表型验证.运用TAIL-PCR技术确定该植株的T-DNA插入位点位于At2g36130,并对该基因的序列进行了初步的生物信息学分析.We determined 90 μmol/L CdCl2 as concentration of screening Cd-sensitive mutant by a concentration gradient assay with Arabidopsis thaliana Col-0.According to the effect of Cd2+ on root growth,seeds from an Arabidopsis mutant library which is characteristic by XVE system were screened.In the process of screening,mutants were firstly screened in 1/2 MS without estrogen,and then the seeds of mutant with better root growth were collected individually.In the second screening of T3,the mutant named csr-2 was identified by the measure of treating with 90 μmol/L Cd2+,and validated by over-expression induce of estrogen.Finally,the insertion site of csr-2 was located at At2g36130 by ail-PCR,and bioinformatics analysis of the gene sequences was conducted initially.

关 键 词：拟南芥 突变体筛选 T-DNA插入突变体 镉 

分 类 号：O943[理学]