siRNA沉默MAPK p42对肝癌SMMC7721增殖和凋亡的影响  

Effects of siRNA-mediated MAPK p42 silence on the proliferation and apoptosis of SMMC7721 cells

作　　者：倪磊[1] 年申[1] 刘利英[1] 姚嘉宜[1] 王爱英[1] 杨娟[1] 胡晓岩[1] 宋土生[1] 

机构地区：[1]西安交通大学医学院遗传学与分子生物学系/环境与疾病相关基因教育部重点实验室生物医学基础研究中心,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2011年第6期677-679,694,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30872483)~~

摘　　要：目的应用siRNA沉默SMMC7721细胞MAPK p42,并观察其对增殖和凋亡的影响。方法应用SilencerTMsiRNA构建试剂盒合成2条MAPK p42siRNAs和一条随机对照siRNAs,用脂质体LipofectaminTM 2000将其转染入肝癌SMMC-7721细胞株。应用Western blot检测MAPK p42表达,MTT法检测细胞增殖情况,流式细胞仪分析细胞周期及其细胞凋亡。结果与对照相比,MAPK p42siRNA明显抑制MAPK p42表达。MAPK p42siRNA抑制了SMMC-7721细胞的生长,将细胞阻滞于细胞周期S期,进而诱导了细胞凋亡的发生。结论 MAPK p42靶向siRNA可能为肝癌基因治疗的重要手段。Objective To explore the effects of siRNA-induced MAPK p42 silence on the proliferation and apoptosis of SMMC7721 cells.Methods Two MAPK p42 siRNAs and one random control siRNA were synthesized by SilencerTM siRNA Construction Kit and transfected into SMMC7721 cells by LipofectaminTM 2000.The expression of MAPK p42 in SMMC7721 cells was analyzed by Western blot,cell growth was tested with MTT,and apoptosis and cell cycle were analyzed by flow cytometry.Results The expression of MAPK p42 was knocked down remarkably in MAPK p42 siRNA-treated cells in comparison with that of negative controls.Cell growth was inhibited,cell cycle was arrested at S phase,and cell apoptosis was induced by MAPK p42 siRNA in SMMC7721 cells.Conclusion Our findings suggest that siRNA-mediated downregulation of MAPK p42 can be an important strategy for cancer therapy.

关 键 词：MAPK P42 SIRNA smmc7721细胞系 细胞凋亡 细胞增殖 

分 类 号：R541[医药卫生—心血管疾病]