结核蛋白芯片对肺结核及肺外结核的诊断价值  

Clinical evaluation of protein microarray chip applied in the diagnosis of pulmonary and extrapulmonary tuberculosis

作　　者：杨群[1,2] 江晓静[1] 

机构地区：[1]广州军区武汉总医院感染病内科,湖北武汉430070 [2]华中科技大学同济医学院附属同济医院感染科,湖北武汉430030

出　　处：《西安交通大学学报（医学版）》2012年第1期129-131,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：湖北省自然科学基金资助项目(No.2010CDB09205)~~

摘　　要：目的通过临床诊断和医学检验报告,评价了结核蛋白芯片在肺结核及肺外结核中的辅助诊断作用。方法应用蛋白芯片对107例肺结核患者及134例肺外结核患者进行结核菌蛋白16ku、38ku和脂阿拉伯甘露糖(LAM)的相应抗体的检测。结果①结核蛋白芯片对结核病的诊断有辅助诊断的价值。②联合三项蛋白芯片抗体指标(16ku蛋白、38ku蛋白、LAM)进行辅助诊断肺结核的价值大于辅助诊断肺外结核的价值,其中肺结核组结核蛋白芯片阳性率为66.4%,肺外结核组阳性率35.8%。③在结核病中三项蛋白芯片抗体指标灵敏性依次为LAM、38ku蛋白、16ku蛋白。④联合蛋白芯片指标灵敏性高于单一蛋白芯片指标。⑤16ku+LAM与16ku+38ku+LAM有近似相同的诊断价值。结论结核蛋白芯片对于检测肺结核和肺外结核有一定的辅助诊断价值。Objective To evaluate the application of protein microarray chip in the diagnosis of pulmonary and extrapulmonary tuberculosis(TB) through clinical diagnosis and medical examination.Methods A total of 107 cases of pulmonary TB and 134 cases of extrapulmonary TB were tested by three kinds of TB antibody detections,including 16 ku,38 ku and LAM.Results ① Tuberculosis protein chip had a diagnostic value for TB.② The diagnostic value of the combined three biomarkers(16 ku protein,32 ku protein and LAM) was greater for pulmonary TB than for extrapulmonary TB.The positive rate for pulmonary TB group and extrapulmonary TB group was 66.4% and 35.8%,respectively.③ In TB,the sensitivity ranking of the three protein chip biomarkers was LAM,32 ku protein and 16 ku protein,respectively.④ The sensitivity of joint protein chip indexes was higher than that of a single protein chip index.⑤ 16 ku+LAM and 16 ku+32 ku+LAM had approximately the same diagnostic value.Conclusion Tuberculosis protein chip has a diagnostic value for pulmonary and extrapulmonary TB.

关 键 词：结核蛋白芯片 肺结核 肺外结核 诊断价值 

分 类 号：R692[医药卫生—泌尿科学]