Characterization of flavonoids in Millettia nitida var. hirsutissima by HPLC/DAD/ESI-MS^n  

作　　者：Min Ye, Wen-Zhi Yang, Ke-Di Liu, Xue Qiao, Bei-Jia Li, Jun Cheng, Jie Feng, De-An Guo, Yu-Ying Zhao State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China 

出　　处：《Journal of Pharmaceutical Analysis》2012年第1期35-42,共8页药物分析学报（英文版）

基　　金：supported by the 985 Project of Peking University Health Science Center (No. 985-2-119-121)

摘　　要：Millettia nitida var. hirsutissima is a Chinese herbal medicine used for the treatment of gynecological diseases. An HPLC/DAD/ESI-MSn method was established for the rapid separation and characterization of bioactive flavonoids in M. nitida var. hirsutissima. A total of 32 flavonoids were detected, of which 14 compounds were unambiguously characterized by comparing their retention time, UV, and MS spectra with those of the reference standards, and the others were tentatively identified based on their tandem mass spectrometry fragmentation data obtained in the negative ionization mode on line. Nineteen of these compounds characterized were reported from this plant for the first time.Millettia nitida var. hirsutissima is a Chinese herbal medicine used for the treatment of gynecological diseases. An HPLC/DAD/ESI-MSn method was established for the rapid separation and characterization of bioactive flavonoids in M. nitida var. hirsutissima. A total of 32 flavonoids were detected, of which 14 compounds were unambiguously characterized by comparing their retention time, UV, and MS spectra with those of the reference standards, and the others were tentatively identified based on their tandem mass spectrometry fragmentation data obtained in the negative ionization mode on line. Nineteen of these compounds characterized were reported from this plant for the first time.

关 键 词：HPLC/DAD/ESI-MSn Millettia nitida var. hirsutissima Mass spectrometry FLAVONOIDS 

分 类 号：R284.1[医药卫生—中药学]