Luminol-potassium permanganate chemiluminescence system for the determination of three anthracycline antibiotics  

作　　者：Hong-Ling Yu,Yu-Hai Tang,Hai-Yan Lü,Jing-Feng Yi Institute of Analytical Sciences,School of Sciences,Xi’an Jiaotong University,Xi’an 710061,China. 

出　　处：《Journal of Pharmaceutical Analysis》2010年第1期20-24,共5页药物分析学报（英文版）

摘　　要：Objective To establish a flow-injection chemiluminescence method for the determination of doxorubicin,epirubicin and mitoxantrone and study its reaction mechanism.Methods In alkaline medium,chemiluminescence of luminol-potassium permanganate system could be inhibited obviously by anthracycline antibiotics.Combined with flow-injection technique,a new chemiluminescence method for determining the anthracycline antibiotics was set up.The chemiluminescence mechanism of the luminol-potassium permanganate system was also discussed.Results Under optimal conditions,the good linear ranges of doxorubicin,epirubicin and mitoxantrone were 5.0×10-9-1.0×10-6g/mL,1.0×10-9-1.0×10-5g/mL and 3×10-9-1.0×10-6g/mL,respectively.The detection limits of doxorubicin,epirubicin and mitoxantrone were 3.0×10-9g/mL,5.0×10-8g/mL and 2.0×10-9g/mL,respectively.During eleven repeated inter-day and intra-day precision tests of 1.0×10-6g/mL samples,the relative standard deviations corresponded to reference values of 3.0%,2.8% and 2.1%.Conclusion The developed method is sensitive,accurate,rapid and of low cost.It can be applied to determine doxorubicin hydrochloride,epirubicin hydrochloride and mitoxantrone hydrochloride in injection preparations.Objective To establish a flow-injection chemiluminescence method for the determination of doxorubicin,epirubicin and mitoxantrone and study its reaction mechanism.Methods In alkaline medium,chemiluminescence of luminol-potassium permanganate system could be inhibited obviously by anthracycline antibiotics.Combined with flow-injection technique,a new chemiluminescence method for determining the anthracycline antibiotics was set up.The chemiluminescence mechanism of the luminol-potassium permanganate system was also discussed.Results Under optimal conditions,the good linear ranges of doxorubicin,epirubicin and mitoxantrone were 5.0×10-9-1.0×10-6g/mL,1.0×10-9-1.0×10-5g/mL and 3×10-9-1.0×10-6g/mL,respectively.The detection limits of doxorubicin,epirubicin and mitoxantrone were 3.0×10-9g/mL,5.0×10-8g/mL and 2.0×10-9g/mL,respectively.During eleven repeated inter-day and intra-day precision tests of 1.0×10-6g/mL samples,the relative standard deviations corresponded to reference values of 3.0%,2.8% and 2.1%.Conclusion The developed method is sensitive,accurate,rapid and of low cost.It can be applied to determine doxorubicin hydrochloride,epirubicin hydrochloride and mitoxantrone hydrochloride in injection preparations.

关 键 词：FLOW-INJECTION CHEMILUMINESCENCE anthracycline antibiotics 

分 类 号：R927[医药卫生—药学]