LC-ESI-MS/MS,a modified method for simultaneous quantification of isoflavonoids,flavonoids,alkaloids and saponins in a Chinese herbal preparation Gegen-Qinlian decoction  

作　　者：Ying Wang,Jin Yuan,Rui An,Xin-Hong Wang,Yue-Ming Ma Department of Chemistry,Shanghai University of Traditional Chinese Medicine School of Pharmacy,Shanghai 201203,China 

出　　处：《Journal of Pharmaceutical Analysis》2010年第3期141-148,共8页药物分析学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.90202039);"Chen Guang" Project for Personnel Cultivation of Shanghai Municipal Education Commission(No.09CG43);the Construction Program for Innovative Research Teamin Shanghai Institutions of Higher Education

摘　　要：A sensitive and reliable liquid chromatography-electrospray ionisation-tandem mass spectrometry(LC-ESI-MS/MS)method was established to simultaneously quantitate four categories of compounds(isoflavonoids,flavonoids,alkaloids and saponins)in Gegen-Qinlian decoction(GQD).These compounds were separated by a Shiseido CAPCELL PAK C18 column with a linear gradient consisting of 0.1%(v/v)formic acid in water(A)and 0.1%(v/v)formic acid in acetonitrile(B),and delivered at a flow rate of 0.3 mL/min.All the analytes were determined by electrospray positive ionization tandem mass spectrometry in a multiple reaction monitoring(MRM)mode.Linearity,accuracy,precision,recovery and stability of the method were evaluated with the validation over the range of 4.0-538 5 ng/mL.The proposed method was applied to the analysis of a Chinese herbal preparation GQD successfully.A sensitive and reliable liquid chromatography-electrospray ionisation-tandem mass spectrometry(LC-ESI-MS/MS)method was established to simultaneously quantitate four categories of compounds(isoflavonoids,flavonoids,alkaloids and saponins)in Gegen-Qinlian decoction(GQD).These compounds were separated by a Shiseido CAPCELL PAK C18 column with a linear gradient consisting of 0.1%(v/v)formic acid in water(A)and 0.1%(v/v)formic acid in acetonitrile(B),and delivered at a flow rate of 0.3 mL/min.All the analytes were determined by electrospray positive ionization tandem mass spectrometry in a multiple reaction monitoring(MRM)mode.Linearity,accuracy,precision,recovery and stability of the method were evaluated with the validation over the range of 4.0-538 5 ng/mL.The proposed method was applied to the analysis of a Chinese herbal preparation GQD successfully.

关 键 词：Gegen-Qinlian decoction(GQD) liquid chromatography-electrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS) quantification 

分 类 号：R284[医药卫生—中药学]