Capillary electrophoresis coupled with electrochemiluminescence for determination of cloperastine hydrochloride  

作　　者：熊凤梅 唐骁爽 唐玉海 木合塔尔.吐尔洪 孙四娟 张广彬 李海燕 

机构地区：[1]Institute of Analytical Sciences, Xi'an Jiaotong University [2]Department of Pharmacy, Baotou Medical College [3]First Affiliated Hospital, Lanzhou University [4]Department of Chemistry, Kashi Teacher's College

出　　处：《Journal of Pharmaceutical Analysis》2008年第4期274-277,共4页药物分析学报（英文版）

摘　　要：Objective To investigate the electrochemiluminescence (ECL) behavior of cloperastine hydrochloride. Methods ECL intensity of tris (2,2′-bipyridyl) rutheniumo(Ⅱ) was enhanced, the method for the determination of cloperastine hydrochloride was established using capillary electrophoresis (CE) coupled with electrochemilumolinescence (ECL) detection. Results Under the optimum conditions, ECL intensity varied linearly with cloperastine hydrochloride concentration from 7.0×10-6g/mL to 1.0×10-4g/mL. The detection limit (S/N=3) was 8.05×10-7g/mL. The relative standard deviation of the ECL intensity and the migration time for 11 consecutive injections of 1.0×10-5g/mL cloperastine hydrochloride was 2.9% and 1.5%, respectively. This method was successfully applied to cloperastine hydrochloride tablet determination. Conclusion The method has been established, validated and applied for determination of cloperastine hydrochloride.Objective To investigate the electrochemiluminescence (ECL) behavior of cloperastine hydrochloride. Methods ECL intensity of tris (2,2′-bipyridyl) rutheniumo(Ⅱ) was enhanced, the method for the determination of cloperastine hydrochloride was established using capillary electrophoresis (CE) coupled with electrochemilumolinescence (ECL) detection. Results Under the optimum conditions, ECL intensity varied linearly with cloperastine hydrochloride concentration from 7.0×10-6g/mL to 1.0×10-4g/mL. The detection limit (S/N=3) was 8.05×10-7g/mL. The relative standard deviation of the ECL intensity and the migration time for 11 consecutive injections of 1.0×10-5g/mL cloperastine hydrochloride was 2.9% and 1.5%, respectively. This method was successfully applied to cloperastine hydrochloride tablet determination. Conclusion The method has been established, validated and applied for determination of cloperastine hydrochloride.

关 键 词：cloperastine hydrochloride capillary electrochemiluminescence tris-(2 2'-bipyridyl) ruthenium (Ⅱ) 

分 类 号：TQ460.72[医药卫生—药物分析学]