Testing Hypotheses of Pleistocene Population History Using Coalescent Simulations: Refugial Isolation and Secondary Contact in Pseudepidalea raddei (Amphibia: Bufonidae)  

作　　者：Bingjun DONG Jing CHE Li DING Song HUANG Robert W.MURPHY Ermi ZHAO Yaping ZHANG 

机构地区：[1]Key Laboratory for Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University [2]College of Chemistry and Life Sciences, Shenyang Normal University [3]State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology, Chinese Academy of Sciences [4]Chengdu Institute of Biology,Chinese Academy of Sciences [5]Department of Biology, Huangshan University [6]Centre for Biodiversity and Conservation Biology, Department of Natural History, Royal Ontario Museum, 100 Queen's Park

出　　处：《Asian Herpetological Research》2012年第2期103-113,共11页亚洲两栖爬行动物研究（英文版）

基　　金：supported by the Director Foundation of Experimental Centre, Shenyang Normal University (Syzx1104);a Visiting Professorship for Senior International Scientists from the Chinese Academy of Sciences to Robert W. MURPHY;supported by a Discovery Grant of the Natural Sciences and Engineering Research Council of Canada (A3148)

摘　　要：The impact of the Quaternary glaciation on eastern China’s local fauna and flora is a topic of considerable interest. We use mitochondrial DNA(mtDNA) sequences and coalescent simulations to test two general biogeographic hypotheses related to the effect of the Pleistocene climatic fluctuations on a widespread, eastern Chinese amphibian, Pseudepidalea raddei. Genealogical reconstructions are made and they detect major western and eastern lineages, which overlap in northwestern China, and possibly indicate the secondary contact of the populations that had entered the region from separate glacial refugia. Coalescent tests rejected alternative hypotheses of fragmentation of either a widespread ancestor or panmixia. The tests instead supported the hypothesis of geographic isolation and a remarkable dispersal pattern in one of the lineages. Though the Pleistocene climatic events are known to have affected the historical distributions and intra-specific divergence of Chinese squamates, coalescent and non-coalescent demographic analyses indicated that the toad P. raddei was not adversely affected by glacial cycling. Presumably, an increase in the amount of climatically mild habitats in East Asia is due to the development of monsoons since the Mid-late Pleistocene is responsible for the relatively mild effects.The impact of the Quaternary glaciation on eastern China’s local fauna and flora is a topic of considerable interest. We use mitochondrial DNA(mtDNA) sequences and coalescent simulations to test two general biogeographic hypotheses related to the effect of the Pleistocene climatic fluctuations on a widespread, eastern Chinese amphibian, Pseudepidalea raddei. Genealogical reconstructions are made and they detect major western and eastern lineages, which overlap in northwestern China, and possibly indicate the secondary contact of the populations that had entered the region from separate glacial refugia. Coalescent tests rejected alternative hypotheses of fragmentation of either a widespread ancestor or panmixia. The tests instead supported the hypothesis of geographic isolation and a remarkable dispersal pattern in one of the lineages. Though the Pleistocene climatic events are known to have affected the historical distributions and intra-specific divergence of Chinese squamates, coalescent and non-coalescent demographic analyses indicated that the toad P. raddei was not adversely affected by glacial cycling. Presumably, an increase in the amount of climatically mild habitats in East Asia is due to the development of monsoons since the Mid-late Pleistocene is responsible for the relatively mild effects.

关 键 词：Mongolian toad Pleistocene refugia PHYLOGEOGRAPHY biogeography 

分 类 号：Q915[天文地球—古生物学与地层学]