Screening and application of microsatellite markers for genetic diversity analysis of Oriental White Stork (Ciconia boyciana)  

东方白鹳微卫星遗传标记的筛选及其在种群遗传分析中的应用(英文)

作　　者：黄赟 周立志[1,2] 

机构地区：[1]安徽大学资源与环境工程学院 [2]安徽大学生物多样性与湿地生态研究所,合肥230601

出　　处：《Chinese Birds》2011年第1期33-38,共6页中国鸟类（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.3087317);the Anhui Academic and Technical Reserve Candidate Leaders Fund

摘　　要：The Oriental White Stork (Ciconia boyciana) is a large and endangered waterbird in East Asia.Research on conservation genetics of this species is urgently needed.In this study,microsatellite marking technology was used for screening and analysis of genetic diversity of microsatellite markers in Oriental White Storks.A total of 36 pairs of microsatellite primers were used,of which 7 pairs came from Ciconia ciconia,12 pairs from Nipponia nippon and 17 pairs from Ardea herodias.Microsatellite loci were screened from 23 individuals of the Oriental White Stork and 11 microsatellite loci were found with high polymorphism.The number of population alleles ranged from 3 to 11,averaging 7.09.The average expected heterozygosity (H e) was 0.7816 and the average polymorphism information content (PIC) 0.7172,suggesting a relatively high genetic diversity in the population.东方白鹳(Ciconia boyciana)是分布于东亚的大型濒危涉禽,亟待深入开展其种群的保护遗传学研究。本研究采用微卫星标记技术筛选东方白鹳的微卫星位点,并进行种群的遗传多样性分析。所用的36对微卫星引物7对来自白鹳(Ciconia ciconia),12对来自朱鹮(Nipponia nippon),17对来自大蓝鹭(Ardea herodias)。从23个东方白鹳个体中筛选获得11个多态性丰富的微卫星位点。群体等位基因数为3-11,平均7.09。平均期望杂合度为0.7816,平均多态信息含量为0.7172,显示出东方白鹳较丰富的种群遗传多样性。

关 键 词：microsatellite molecular marker Ciconia boyciana genetic diversity 

分 类 号：Q953[生物学—动物学]