大黄对小鼠急性肝损伤蛋白质表达的影响  

Effects of rhubarb on protein expression of acute liver injury of mice

作　　者：田种存[1] 靳智勇[2] 

机构地区：[1]青海大学农林科学院,青海西宁810016 [2]青海大学畜牧兽医科学院,青海西宁810016

出　　处：《青海大学学报（自然科学版）》2012年第3期43-45,共3页Journal of Qinghai University(Natural Science)

摘　　要：以大黄为研究对象,将CCl4急性肝损伤模型组、空白对照组和大黄高剂量、中剂量、低剂量等药物组进行处理,小鼠断头处死取其肝脏研究大黄对小鼠肝脏蛋白质的影响。实验运用SDS聚丙烯酰胺凝胶电泳法分析大黄对各组实验小鼠肝脏蛋白质的损伤。结果表明,在大黄作用下,蛋白质的表达有所变化,表现在高剂量组有新增加的蛋白的出现,同时蛋白含量有所变化,在中低剂量组中更加明显,并表明CCl4肝损伤模型可引起转氨酶升高,而大黄有降酶作用,得出大黄对CCl4化学性肝损伤具有保护作用。CCl4 acute liver injury model group,check group and rhubarb treatment group(high-,moderate-and Iow-dose) were used to investigate the effect of rhubarb on mice liver protein expression.Electrophoresis analysis revealed that rhubarb may cause liver protein expression change of mice.High-dose group presented new protein and increased protein content,and moderate and low dose group exhibited obvious change.CCl4 may cause transaminase increase and rhubarb decreased transaminase.Rhubarb can protect liver injury caused by CCl4.

关 键 词：大黄 CCl4急性肝损伤小鼠 蛋白质电泳 

分 类 号：R28[医药卫生—中药学]