半夏种质资源的高效毛细管电泳(HPCE)分析  

The analysis of Pinellia germplasm resources by high performance capillary electrophoresis

作　　者：卢道会[1] 李敏[1] 杨小艳[1] 齐景梁[1] 石万银[1] 

机构地区：[1]成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075

出　　处：《中药与临床》2012年第4期7-9,26,共4页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省"十二五"农作物及畜禽育种攻关--川产道地药材育种新材料及新方法创制

摘　　要：目的:对半夏蛋白的组成情况进行初步的探讨和分析,了解半夏种质资源的差异。方法:利用毛细管电泳提供的蛋白信息,采用SPSS主成分分析法对半夏的蛋白组分进行分析,通过聚类分析结果了解半夏种质资源之间的关系。结果:根据高效毛细管电泳的信息,通过主成分分析和聚类分析,对半夏种子资源进行分类比较,结果表明半夏蛋白的组成与其地域分布有一定的关系。结论:不同来源的半夏种质资源之间存在一定的亲缘关系,可为半夏优良品种的筛选奠定基础。Objective:Germplasm resources difference of Pinellia was investigated by preliminary study and analysis of the protein composition.Method:The protein of Pinellia was analyzed by the capillary electrophoresis,and the results were analyzed using principal component analysis,and the relations of germplasm resources were analyzed using cluster analysis.Result:The different populations of Pinellia resources were compared by principal component analysis and the cluster analysis.The results showed that the composition of its protein was relation to the geographical distribution.Conclusion:Genetic relationship can be found in Pinellia of different germplasm resources which can provide the reference for screening of fine varieties.

关 键 词：半夏 种质资源 毛细管电泳 主成分分析 聚类分析 

分 类 号：R28[医药卫生—中药学]